Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SYND3/SDC3/Syndecan-3 CRISPR/Cas9 KO Plasmid (m): sc-423236
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SYND3/SDC3/Syndecan-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SYND3/SDC3/Syndecan-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SYND3/SDC3/Syndecan-3 Antibody (G-2): sc-398194
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SYND3/SDC3/Syndecan-3 CRISPR/Cas9 KO Plasmid (m) | sc-423236 | 20 µg | $397.00 |
Overview
Sdc3 encodes syndecan-3 (SYND3/SDC3), a transmembrane heparan sulfate proteoglycan that organizes extracellular matrix cues and growth factor gradients at the cell surface. By binding heparan sulfate–dependent ligands, SDC3 modulates receptor tyrosine kinase and chemokine signaling, influencing cytoskeletal remodeling, cell migration, neurite outgrowth, and synaptic plasticity. In mouse, Sdc3 is prominent in neuronal and metabolic tissues and contributes to pathways governing axon guidance, cell–matrix adhesion, and inflammatory signaling. Dysregulated syndecan-3 function has been associated with neurodevelopmental and neuroinflammatory processes and with altered energy balance phenotypes, supporting its use as a mechanistic node in CNS and metabolism research.
SYND3/SDC3/Syndecan-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sdc3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sdc3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sdc3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SYND3/SDC3/Syndecan-3 protein expression.
This CRISPR knockout system enables efficient generation of Sdc3-deficient cell models for investigation of SYND3/SDC3/Syndecan-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sdc3 exon(s) critical for SYND3/SDC3/Syndecan-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sdc3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SYND3/SDC3/Syndecan-3 CRISPR/Cas9 KO Plasmid (m) and SYND3/SDC3/Syndecan-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sdc3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SYND3/SDC3/Syndecan-3 HDR Plasmid (m) and SYND3/SDC3/Syndecan-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sdc3 homology arms to support homology-directed repair at defined Sdc3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.