Syncoilin CRISPR/Cas9 KO Plasmid (h): sc-406493
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Syncoilin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Syncoilin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Syncoilin Antibody (C-3): sc-515474
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Syncoilin CRISPR/Cas9 KO Plasmid (h) | sc-406493 | 20 µg | $397.00 |
Overview
Human SYNC encodes syncoilin, an intermediate filament–associated protein enriched in skeletal and cardiac muscle that localizes to the sarcolemma and Z-disc regions. Syncoilin participates in cytoskeletal organization by linking intermediate filament networks to dystrophin-associated protein complexes, supporting myofiber integrity and mechanotransduction during contraction. Through these interactions it contributes to myogenic differentiation, sarcolemmal stability, and maintenance of muscle architecture under mechanical stress. Altered SYNC expression and syncoilin mislocalization have been reported in muscle pathology contexts, making it relevant for studies of myopathy-associated cytoskeletal remodeling and stress-response pathways.
Syncoilin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SYNC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SYNC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SYNC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Syncoilin protein expression.
This CRISPR knockout system enables efficient generation of SYNC-deficient cell models for investigation of Syncoilin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SYNC exon(s) critical for Syncoilin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SYNC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Syncoilin CRISPR/Cas9 KO Plasmid (h) and Syncoilin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SYNC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Syncoilin HDR Plasmid (h) and Syncoilin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SYNC homology arms to support homology-directed repair at defined SYNC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.