Synaptotagmin XI Antibody (D-5): sc-365991

Synaptotagmin XI Antibody (D-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Synaptotagmin XI protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Synaptotagmin XI antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Synaptotagmin XI, also known as SYT11, is a 431 amino acid protein that plays a crucial role in synaptic vesicle trafficking and neurotransmitter release. SYT11 is primarily localized to the membrane and is expressed ubiquitously, with highest levels found in brain and lung. Notably, SYT11 is suggested to be calcium-independent due to a single point mutation in one of its C2 domains, which distinguishes SYT11 from other members of the synaptotagmin family that typically function as calcium sensors. This unique characteristic may influence SYT11′s interactions and regulatory roles in synaptic processes. Additionally, SYT11 interacts with ubiquitin-E3-ligase Parkin, a gene product associated with juvenile Parkinson′s disease, leading to polyubiquitination and subsequent degradation of SYT11 by the proteasome complex. Defects in the gene encoding SYT11 have been implicated in various neurological disorders, including schizophrenia and Parkinson′s disease, highlighting SYT11′s importance in maintaining synaptic integrity and function.

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Synaptotagmin XI Antibody (D-5) References:

1. SYNCRIP, a cytoplasmic counterpart of heterogeneous nuclear ribonucleoprotein R, interacts with ubiquitous synaptotagmin isoforms.  |  Mizutani, A., et al. 2000. J Biol Chem. 275: 9823-31. PMID: 10734137
2. Genomic analysis of synaptotagmin genes.  |  Craxton, M. 2001. Genomics. 77: 43-9. PMID: 11543631
3. The autosomal recessive juvenile Parkinson disease gene product, parkin, interacts with and ubiquitinates synaptotagmin XI.  |  Huynh, DP., et al. 2003. Hum Mol Genet. 12: 2587-97. PMID: 12925569
4. Screening for mutations in synaptotagmin XI in Parkinson's disease.  |  Glass, AS., et al. 2004. J Neural Transm Suppl. 21-8. PMID: 15354386
5. Effects of methylation of non-CpG sequence in the promoter region on the expression of human synaptotagmin XI (syt11).  |  Inoue, S. and Oishi, M. 2005. Gene. 348: 123-34. PMID: 15777718
6. Synaptotagmin XI as a candidate gene for susceptibility to schizophrenia.  |  Inoue, S., et al. 2007. Am J Med Genet B Neuropsychiatr Genet. 144B: 332-40. PMID: 17192956
7. The functional significance of synaptotagmin diversity in neuroendocrine secretion.  |  Moghadam, PK. and Jackson, MB. 2013. Front Endocrinol (Lausanne). 4: 124. PMID: 24065953
8. The evolutionary pressure to inactivate. A subclass of synaptotagmins with an amino acid substitution that abolishes Ca2+ binding.  |  von Poser, C., et al. 1997. J Biol Chem. 272: 14314-9. PMID: 9162066
9. Calcium-dependent oligomerization of synaptotagmins I and II. Synaptotagmins I and II are localized on the same synaptic vesicle and heterodimerize in the presence of calcium.  |  Osborne, SL., et al. 1999. J Biol Chem. 274: 59-66. PMID: 9867811

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Synaptotagmin XI Antibody (D-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Synaptotagmin XI Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Synaptotagmin XI Antibody (D-5) Specifications

Alternate Product Name: Synaptotagmin XI

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 353-431

Epitope Target: Synaptotagmin XI

Epitope Species: human

Product Reactivity/Detection: Synaptotagmin XI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Synaptotagmin XI Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Synaptotagmin XI antibody is Synaptotagmin XI Antibody (H-7): sc-515632.