Synaptotagmin VI Antibody (A-12): sc-390321

Synaptotagmin VI Antibody (A-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Synaptotagmin VI in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Synaptotagmin VI antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Synaptotagmin VI is a member of the synaptotagmin family, playing a crucial role in regulating synaptic vesicle exocytosis and endocytosis, facilitating neurotransmitter release from secretory vesicles. Synaptotagmin VI is primarily located in presynaptic terminals of neurons, acting as a calcium sensor that triggers neurotransmitter release upon calcium influx. This localization ensures neurotransmitter release in response to neuronal activity, influencing communication between neurons. Synaptotagmin VI has also shown interaction with adaptor protein-2 in a calcium-independent manner, highlighting a role in complex mechanisms of synaptic function and plasticity. Understanding Synaptotagmin VI function and localization helps elucidate contributions to both normal synaptic physiology and potential pathophysiological conditions, such as neurological disorders.

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Synaptotagmin VI Antibody (A-12) References:

1. Role of synaptotagmin, a Ca2+ and inositol polyphosphate binding protein, in neurotransmitter release and neurite outgrowth.  |  Mikoshiba, K., et al. 1999. Chem Phys Lipids. 98: 59-67. PMID: 10358928
2. The subcellular localizations of atypical synaptotagmins III and VI. Synaptotagmin III is enriched in synapses and synaptic plasma membranes but not in synaptic vesicles.  |  Butz, S., et al. 1999. J Biol Chem. 274: 18290-6. PMID: 10373432
3. A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms.  |  Fukuda, M. and Mikoshiba, K. 1999. J Biol Chem. 274: 31428-34. PMID: 10531344
4. Synaptotagmin VI participates in the acrosome reaction of human spermatozoa.  |  Michaut, M., et al. 2001. Dev Biol. 235: 521-9. PMID: 11437455
5. alpha-SNAP and NSF are required in a priming step during the human sperm acrosome reaction.  |  Tomes, CN., et al. 2005. Mol Hum Reprod. 11: 43-51. PMID: 15542541
6. Protein kinase C-mediated phosphorylation of the two polybasic regions of synaptotagmin VI regulates their function in acrosomal exocytosis.  |  Roggero, CM., et al. 2005. Dev Biol. 285: 422-35. PMID: 16111671
7. Complexin/synaptotagmin interplay controls acrosomal exocytosis.  |  Roggero, CM., et al. 2007. J Biol Chem. 282: 26335-43. PMID: 17613520
8. PTP1B dephosphorylates N-ethylmaleimide-sensitive factor and elicits SNARE complex disassembly during human sperm exocytosis.  |  Zarelli, VE., et al. 2009. J Biol Chem. 284: 10491-503. PMID: 19208619
9. Calcineurin-mediated dephosphorylation of synaptotagmin VI is necessary for acrosomal exocytosis.  |  Castillo Bennett, J., et al. 2010. J Biol Chem. 285: 26269-78. PMID: 20551332
10. Inositol 1,3,4,5-tetrakisphosphate binding activities of neuronal and non-neuronal synaptotagmins. Identification of conserved amino acid substitutions that abolish inositol 1,3,4,5-tetrakisphosphate binding to synaptotagmins III, V, and X.  |  Ibata, K., et al. 1998. J Biol Chem. 273: 12267-73. PMID: 9575177

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Synaptotagmin VI Antibody (A-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Synaptotagmin VI Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Synaptotagmin VI Antibody (A-12) Specifications

Alternate Product Name: Synaptotagmin VI

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 84-137

Epitope Target: Synaptotagmin VI

Epitope Species: human

Product Reactivity/Detection: Synaptotagmin VI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Synaptotagmin VI Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Synaptotagmin VI antibody is Synaptotagmin VI Antibody (A-5): sc-390320.