Synaptotagmin VI Antibody (A-5): sc-390320

Synaptotagmin VI Antibody (A-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Synaptotagmin VI protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Synaptotagmin VI antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Synaptotagmin VI is part of a large family of synaptic vesicle proteins that play a crucial role in regulating exocytosis and endocytosis, which are essential processes for neurotransmitter release from small secretory vesicles. Synaptotagmin VI is primarily located in presynaptic terminals of neurons, where Synaptotagmin VI acts as a calcium sensor triggering neurotransmitter release upon calcium influx. This localization enables proper synaptic transmission by ensuring neurotransmitter release in response to neuronal activity, facilitating communication between neurons. Synaptotagmin VI has also been shown to interact with various proteins, including adaptor protein-2, in a calcium-independent manner, highlighting Synaptotagmin VI′s role in synaptic vesicle trafficking and neurotransmitter release regulation. Understanding Synaptotagmin VI function and localization helps elucidate mechanisms underlying synaptic transmission and implications in neurological disorders.

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Synaptotagmin VI Antibody (A-5) References:

1. Analysis of synaptotagmin I-IV messenger RNA expression and developmental regulation in the rat hypothalamus and pituitary.  |  Xi, D., et al. 1999. Neuroscience. 88: 425-35. PMID: 10197764
2. Role of synaptotagmin, a Ca2+ and inositol polyphosphate binding protein, in neurotransmitter release and neurite outgrowth.  |  Mikoshiba, K., et al. 1999. Chem Phys Lipids. 98: 59-67. PMID: 10358928
3. The human synaptotagmin IV gene defines an evolutionary break point between syntenic mouse and human chromosome regions but retains ligand inducibility and tissue specificity.  |  Ferguson, GD., et al. 2000. J Biol Chem. 275: 36920-6. PMID: 10938284
4. Synaptotagmin VI participates in the acrosome reaction of human spermatozoa.  |  Michaut, M., et al. 2001. Dev Biol. 235: 521-9. PMID: 11437455
5. SNARE complex assembly is required for human sperm acrosome reaction.  |  Tomes, CN., et al. 2002. Dev Biol. 243: 326-38. PMID: 11884041
6. Protein kinase C-mediated phosphorylation of the two polybasic regions of synaptotagmin VI regulates their function in acrosomal exocytosis.  |  Roggero, CM., et al. 2005. Dev Biol. 285: 422-35. PMID: 16111671
7. Complexin/synaptotagmin interplay controls acrosomal exocytosis.  |  Roggero, CM., et al. 2007. J Biol Chem. 282: 26335-43. PMID: 17613520
8. PTP1B dephosphorylates N-ethylmaleimide-sensitive factor and elicits SNARE complex disassembly during human sperm exocytosis.  |  Zarelli, VE., et al. 2009. J Biol Chem. 284: 10491-503. PMID: 19208619
9. Calcineurin-mediated dephosphorylation of synaptotagmin VI is necessary for acrosomal exocytosis.  |  Castillo Bennett, J., et al. 2010. J Biol Chem. 285: 26269-78. PMID: 20551332
10. Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins.  |  Li, C., et al. 1995. Nature. 375: 594-9. PMID: 7791877
11. A third synaptotagmin gene, Syt3, in the mouse.  |  Hilbush, BS. and Morgan, JI. 1994. Proc Natl Acad Sci U S A. 91: 8195-9. PMID: 8058779
12. Expression of synaptotagmin VIII in rat kidney.  |  Kishore, BK., et al. 1998. Am J Physiol. 275: F131-42. PMID: 9689015

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Synaptotagmin VI Antibody (A-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Synaptotagmin VI Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Synaptotagmin VI Antibody (A-5) Specifications

Alternate Product Name: Synaptotagmin VI

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 84-137

Epitope Target: Synaptotagmin VI

Epitope Species: human

Product Reactivity/Detection: Synaptotagmin VI

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Synaptotagmin VI Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Synaptotagmin VI antibody is Synaptotagmin VI Antibody (A-12): sc-390321.