Synaptotagmin I/II Antibody (H-9): sc-393392

Synaptotagmin I/II Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Synaptotagmin I/II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Synaptotagmin I/II antibody (H-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Synaptotagmins are integral membrane proteins that play a crucial role in regulating synaptic transmission, specifically in exocytosis and endocytosis processes. Synaptotagmin I acts as a calcium sensor, binding calcium ions to trigger neurotransmitter release at synapses, facilitating communication between neurons. Synaptotagmin II undergoes phosphorylation by WNK1, which modulates calcium-dependent interactions, highlighting post-translational modifications′ significance in functional regulation. A diverse family of synaptotagmins, including Synaptotagmin III, IV, V, and VII, demonstrates essential roles in various cellular contexts, including endocrine cells and neuronal tissues, with potential implications in psychiatric disorders due to brain expression patterns.

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Synaptotagmin I/II Antibody (H-9) References:

1. Analysis of synaptotagmin I-IV messenger RNA expression and developmental regulation in the rat hypothalamus and pituitary.  |  Xi, D., et al. 1999. Neuroscience. 88: 425-35. PMID: 10197764
2. The human synaptotagmin IV gene defines an evolutionary break point between syntenic mouse and human chromosome regions but retains ligand inducibility and tissue specificity.  |  Ferguson, GD., et al. 2000. J Biol Chem. 275: 36920-6. PMID: 10938284
3. Distribution of the presynaptic calcium sensors, synaptotagmin I/II and synaptotagmin III, in the goldfish and rodent retinas.  |  Berntson, AK. and Morgans, CW. 2003. J Vis. 3: 274-80. PMID: 12803536
4. A proteomic survey of rat cerebral cortical synaptosomes.  |  Witzmann, FA., et al. 2005. Proteomics. 5: 2177-201. PMID: 15852343
5. Two Feet on the Membrane: Uptake of Clostridial Neurotoxins.  |  Rummel, A. 2017. Curr Top Microbiol Immunol. 406: 1-37. PMID: 27921176
6. Cellular localization of synaptotagmin I, II, and III mRNAs in the central nervous system and pituitary and adrenal glands of the rat.  |  Marquèze, B., et al. 1995. J Neurosci. 15: 4906-17. PMID: 7623121
7. Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins.  |  Li, C., et al. 1995. Nature. 375: 594-9. PMID: 7791877
8. A third synaptotagmin gene, Syt3, in the mouse.  |  Hilbush, BS. and Morgan, JI. 1994. Proc Natl Acad Sci U S A. 91: 8195-9. PMID: 8058779
9. Synaptotagmin III is a novel isoform of rat synaptotagmin expressed in endocrine and neuronal cells.  |  Mizuta, M., et al. 1994. J Biol Chem. 269: 11675-8. PMID: 8163462
10. Developmental regulation of synaptotagmin I, II, III, and IV mRNAs in the rat CNS.  |  Berton, F., et al. 1997. J Neurosci. 17: 1206-16. PMID: 9006966
11. Exocytotic proteins in enterochromaffin-like (ECL) cells of the rat stomach.  |  Zhao, CM., et al. 1997. Cell Tissue Res. 290: 539-51. PMID: 9369530
12. Expression of synaptotagmin VIII in rat kidney.  |  Kishore, BK., et al. 1998. Am J Physiol. 275: F131-42. PMID: 9689015

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Synaptotagmin I/II Antibody (H-9) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-Synaptotagmin I/II Antibody (H-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Synaptotagmin I/II Antibody (H-9) Specifications

Alternate Product Name: Synaptotagmin I/II

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 301-318

Epitope Target: Synaptotagmin I

Epitope Species: human

Product Reactivity/Detection: Synaptotagmin I/II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for Synaptotagmin I/II Antibody (H-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin