Syk Antibody (4D10): sc-1240

Syk Antibody (4D10) is a mouse monoclonal IgG2a kappa light chain antibody that detects Syk protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin embedded sections (IHCP), and flow cytometry (FCM). Syk (4D10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Syk protein, consisting of 635 amino acids, features one protein kinase domain and two SH2 domains, positioning Syk as a crucial member of the protein kinase superfamily. Syk plays a vital role in B cell signaling by acting as a positive effector of B cell antigen receptor (CD79)-stimulated responses, facilitating calcium ion movement through two distinct phospho-regulated pathways. When unphosphorylated, calcium ions traverse a phosphoinositide 3-kinase-dependent pathway, while phosphorylation at tyrosine residues 348 and 352 shifts calcium flow to a phospholipase C gamma-dependent pathway. This regulatory mechanism is essential for various cellular processes, including differentiation, phagocytosis, proliferation, and B cell development. Syk expression is upregulated in T cell lymphoma, indicating potential involvement in tumorigenesis. Syk exists in two isoforms, short and long, resulting from alternative splicing events, which may contribute to functional diversity in cellular signaling pathways.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Syk Antibody (4D10) References:

1. Association of the 72-kDa protein-tyrosine kinase PTK72 with the B cell antigen receptor.  |  Hutchcroft, JE., et al. 1992. J Biol Chem. 267: 8613-9. PMID: 1569106
2. Syk is downstream of intercellular adhesion molecule-1 and mediates human rhinovirus activation of p38 MAPK in airway epithelial cells.  |  Wang, X., et al. 2006. J Immunol. 177: 6859-70. PMID: 17082600
3. Deregulated Syk inhibits differentiation and induces growth factor-independent proliferation of pre-B cells.  |  Wossning, T., et al. 2006. J Exp Med. 203: 2829-40. PMID: 17130299
4. Tyrosine kinase Syk associates with toll-like receptor 4 and regulates signaling in human monocytic cells.  |  Chaudhary, A., et al. 2007. Immunol Cell Biol. 85: 249-56. PMID: 17228323
5. CD303 (BDCA-2) signals in plasmacytoid dendritic cells via a BCR-like signalosome involving Syk, Slp65 and PLCgamma2.  |  Röck, J., et al. 2007. Eur J Immunol. 37: 3564-75. PMID: 18022864
6. Activation of Syk by protein kinase C-delta regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via tyrosine phosphorylation of RelA/p65.  |  Bijli, KM., et al. 2008. J Biol Chem. 283: 14674-84. PMID: 18362147
7. Overexpression of Syk tyrosine kinase in peripheral T-cell lymphomas.  |  Feldman, AL., et al. 2008. Leukemia. 22: 1139-43. PMID: 18401419
8. Hepatitis C virus NS5A protein interacts with and negatively regulates the non-receptor protein tyrosine kinase Syk.  |  Inubushi, S., et al. 2008. J Gen Virol. 89: 1231-1242. PMID: 18420802
9. Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis: a multi-omics study with drug exploration.  |  Wang, M., et al. 2022. Signal Transduct Target Ther. 7: 157. PMID: 35551173
10. SYK coordinates neuroprotective microglial responses in neurodegenerative disease.  |  Ennerfelt, H., et al. 2022. Cell. 185: 4135-4152.e22. PMID: 36257314
11. TREM2 drives microglia response to amyloid-β via SYK-dependent and -independent pathways.  |  Wang, S., et al. 2022. Cell. 185: 4153-4169.e19. PMID: 36306735
12. Syk protein-tyrosine kinase is regulated by tyrosine-phosphorylated Ig alpha/Ig beta immunoreceptor tyrosine activation motif binding and autophosphorylation.  |  Rowley, RB., et al. 1995. J Biol Chem. 270: 11590-4. PMID: 7538118

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Syk Antibody (4D10) has 269 citations in a variety of scientific publications.

Primary antibodies like Anti-Syk Antibody (4D10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Syk Antibody (4D10) Specifications

Alternate Product Name: Syk

Product Clone Name: 4D10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 313-339

Epitope Target: Syk

Epitope Species: human

Product Reactivity/Detection: Syk

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 269 citations in various scientific publications

Available Conjugates/Forms for Syk Antibody (4D10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin