SV40 T Ag Antibody (LP7N): sc-53447
- SV40 T Ag Antibody (LP7N) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against detergent insoluble fraction of SVK14 cell line
- recommended for detection of large T antigen of SV40 origin by WB, IP and IF
- See SV40 T Ag (Pab 101): sc-147 for SV40 T Ag antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for SV40 T Ag Antibody (LP7N) for WB applications. This reagent is now offered in a bundle with SV40 T Ag Antibody (LP7N) (see ordering information below).
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SV40 T Ag Antibody (LP7N) is a mouse monoclonal IgG1 antibody that detects SV40 T Ag of SV40 species reactivity by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). SV40 T Ag plays a crucial role in the viral life cycle and is essential for the transformation of host cells, as SV40 T Ag interacts with key cellular proteins that regulate cell growth and division. Notably, SV40 T Ag binds to the retinoblastoma (Rb) protein, specifically targeting the "pocket" domain, which is also the binding site for the E2F transcription factor. This interaction leads to the inactivation of Rb, disrupting Rb′s ability to regulate the cell cycle and promoting uncontrolled cell proliferation. Additionally, SV40 T Ag′s ability to form complexes with other tumor suppressor proteins, such as p53, further underscores SV40 T Ag′s importance in oncogenic processes. Anti-SV40 T Ag antibody (LP7N) serves as an invaluable tool for researchers studying the mechanisms of viral oncogenesis and the intricate pathways involved in cellular transformation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SV40 T Ag Antibody (LP7N) References:
- SV40 T-antigen-binding sites within the 5'-flanking regions of human U1 and U2 genes. | Han, YM., et al. 1991. Gene. 109: 219-31. PMID: 1662655
- SV40 T-antigen is required for maintenance of immortal growth in SV40-transformed human fibroblasts. | Tsuyama, N., et al. 1991. Cell Struct Funct. 16: 55-62. PMID: 1851674
- SV40 T antigen disrupted the cell metabolism and the balance between proliferation and apoptosis in lens tumors of transgenic mice. | Zheng, HC., et al. 2009. J Cancer Res Clin Oncol. 135: 1521-32. PMID: 19466455
- Characterization of SV40 T antigen associated with the nuclear matrix. | Mann, K. 1990. Virology. 179: 437-45. PMID: 2171215
- Binding studies of SV40 T-antigen to SV40 binding site II. | Gottlieb, P., et al. 1985. Nucleic Acids Res. 13: 6621-34. PMID: 2997717
- A model for polyomavirus helicase activity derived in part from the AlphaFold2 structure of SV40 T-antigen. | Shin, J., et al. 2024. J Virol. 98: e0111924. PMID: 39311578
- Binding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants. | McKay, R. and DiMaio, D. 1981. Nature. 289: 810-3. PMID: 6258090
- Functional interactions between SV40 T antigen and other replication proteins at the replication fork. | Murakami, Y. and Hurwitz, J. 1993. J Biol Chem. 268: 11008-17. PMID: 8098707
- Simian virus 40 (SV40) T-antigen mutations in tumorigenic transformation of SV40-immortalized human uroepithelial cells. | Kao, C., et al. 1993. J Virol. 67: 1987-95. PMID: 8383222
- p53 and SV40 T antigen bind to the same region overlapping the conserved domain of the TATA-binding protein. | Martin, DW., et al. 1993. Biochem Biophys Res Commun. 195: 428-34. PMID: 8395834
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SV40 T Ag Antibody (LP7N) | sc-53447 | 200 µg/ml | $322.00 | |||
SV40 T Ag Antibody (LP7N): m-IgG Fc BP-HRP Bundle | sc-538991 | 200 µg Ab; 10 µg BP | $361.00 |