SV40 T Ag Antibody (H-1): sc-25326

SV40 T Ag Antibody (H-1) is a mouse monoclonal IgG2a antibody that detects SV40 T Ag in SV40 through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SV40 T Ag plays a crucial role in the viral life cycle and is essential for the transformation of host cells, making SV40 T Ag a significant focus in cancer research. SV40 T Ag is primarily located in the nucleus of infected cells, where SV40 T Ag exerts effects by interacting with key cellular proteins involved in cell cycle regulation. Notably, SV40 T Ag binds to the retinoblastoma (Rb) tumor suppressor protein, specifically targeting the "pocket" domain, which is also the binding site for the E2F transcription factor. This interaction leads to the inactivation of Rb, resulting in the release of E2F and subsequent transcription of genes that promote cell proliferation. Understanding the nuclear localization and function of SV40 T Ag is vital for elucidating the mechanisms of viral oncogenesis and the broader implications for tumor biology. Anti-SV40 T Ag antibody (H-1) is an invaluable tool for researchers investigating these pathways and their potential therapeutic targets.

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SV40 T Ag Antibody (H-1) References:

1. The C3(1)/SV40 T-antigen transgenic mouse model of mammary cancer: ductal epithelial cell targeting with multistage progression to carcinoma.  |  Green, JE., et al. 2000. Oncogene. 19: 1020-7. PMID: 10713685
2. Preformed hexamers of SV40 T antigen are active in RNA and origin-DNA unwinding.  |  Uhlmann-Schiffler, H., et al. 2002. Nucleic Acids Res. 30: 3192-201. PMID: 12136101
3. SV40 T antigen interacts with Nbs1 to disrupt DNA replication control.  |  Wu, X., et al. 2004. Genes Dev. 18: 1305-16. PMID: 15175262
4. Effects of SV40 T antigen transduction on human corneal endothelial cell wound healing in vitro.  |  Hsiao, CH., et al. 2005. J Ocul Pharmacol Ther. 21: 353-66. PMID: 16245961
5. SV40 T antigen alone drives karyotype instability that precedes neoplastic transformation of human diploid fibroblasts.  |  Ray, FA., et al. 1990. J Cell Biochem. 42: 13-31. PMID: 2153691
6. SV40 T-antigen expression in cultured fibroblasts from patients with Down syndrome and their parents.  |  Lubiniecki, AS., et al. 1979. Am J Hum Genet. 31: 469-77. PMID: 225951
7. HAdV-2-suppressed growth of SV40 T antigen-transformed mouse mammary epithelial cell-induced tumours in SCID mice.  |  Wu, C., et al. 2016. Virology. 489: 44-50. PMID: 26707269
8. Purification of SV40 T-antigen by SV40 DNA-sepharose affinity chromatography.  |  Milavetz, B., et al. 1986. Biochem Biophys Res Commun. 134: 915-21. PMID: 3004472
9. Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region.  |  Bae, DH., et al. 2020. Mol Ther Methods Clin Dev. 18: 631-638. PMID: 32775497
10. Pituitary Tumors and Immortalized Cell Lines Generated by Cre-Inducible Expression of SV40 T Antigen.  |  Daly, AZ., et al. 2021. Endocrinology. 162: PMID: 33837405

The preferred SV40 T Ag antibody is SV40 T Ag Antibody (Pab 101): sc-147.