SV40 T Ag Antibody (H-1): sc-25326

SV40 T Ag Antibody (H-1) is a mouse monoclonal IgG2a antibody that detects SV40 T Ag in SV40 through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SV40 T Ag plays a crucial role in the viral life cycle and is essential for the transformation of host cells, making SV40 T Ag a significant focus in cancer research. SV40 T Ag is primarily located in the nucleus of infected cells, where SV40 T Ag exerts effects by interacting with key cellular proteins involved in cell cycle regulation. Notably, SV40 T Ag binds to the retinoblastoma (Rb) tumor suppressor protein, specifically targeting the "pocket" domain, which is also the binding site for the E2F transcription factor. This interaction leads to the inactivation of Rb, resulting in the release of E2F and subsequent transcription of genes that promote cell proliferation. Understanding the nuclear localization and function of SV40 T Ag is vital for elucidating the mechanisms of viral oncogenesis and the broader implications for tumor biology. Anti-SV40 T Ag antibody (H-1) is an invaluable tool for researchers investigating these pathways and their potential therapeutic targets.

The preferred SV40 T Ag antibody is SV40 T Ag Antibody (Pab 101): sc-147.