survivin Antibody (D-8) recommended for detection of survivin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

survivin Antibody (D-8): sc-17779

survivin Antibody (D-8) is rated 4.5 out of 5 by 36.
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Datasheets

    • Anti-survivin Antibody (D-8) is a mouse monoclonal IgG2a κ survivin antibody, cited in 331 publications, provided at 200 µg/ml
    • raised against amino acids 1-142 of survivin of human origin
    • Anti-survivin Antibody (D-8) is recommended for detection of survivin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-survivin Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of survivin (D-8): sc-17779.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for survivin Antibody (D-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with survivin Antibody (D-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I am doing research in Medulloblastoma. I need survivin antibody (IHC ). Please send details about survivin staining procedures and it's cost.

Asked by: Gural G
Thank you for your question. Please contact customer care with your address details so we can send you a quote for this product. You can find our standard IHC protocol under this link: https://www.scbt.com/scbt/de/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2020-12-31

I´d like to know if this antibody can be used for FCM conjugated with PE. If it is possible, how is the best concentration?

Asked by: MariaCSS
Thank you for your question. Yes, this mouse monoclonal antibody, survivin (D-8): sc-17779, is available conjugated to phycoerythrin (sc-17779 PE) 200 µg/ml for flow cytometry (FCM). We recommend using 1 µg of conjugated primary antibody for every 100 µl of the prepared cell suspension (equal to 1 million cells). Please refer to the link below for our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-09-14

Is it possible for the survivin Antibody (D-8) to work in bovine samples?

Asked by: Trav11
To check additional species reactivity not listed on our website, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-20

I would like to perform double or triple staining, but my other primary antibodies are also from mice. Is this possible?

Asked by: Trav11
Yes, this is absolutely possible. In this case, we just recommend using our mouse monoclonal primary antibodies that are directly conjugated. Currently, the survivin Antibody (D-8): sc-17779 is available conjugated to HRP (sc-17779 HRP), phycoerythrin (sc-17779 PE), fluoroscein (sc-17779 FITC), Alexa Fluor® 488 (sc-17779 AF488) or Alexa Fluor® 647 (sc-17779 AF647).
Answered by: Tech Service 8
Date published: 2016-12-20
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Rated 5 out of 5 by from Good product it's good for Western blot ,this antibody is very useful.
Date published: 2019-10-16
Rated 1 out of 5 by from Did not work for WB analysis Tried at a 1:500 dilution in human and canine bladder cancer cells but detected no signal.
Date published: 2018-06-19
Rated 5 out of 5 by from survivin 申请试用装后结果很好,于是购买了正品!santa 的抗体如果实验结果不错的话,还是要推荐的,毕竟性价比很高。
Date published: 2018-05-25
Rated 4 out of 5 by from ottime bande Ho ricevuto un free trial di questo anticorpo che ho provato su lisato totale della linea di glioma umano U373-MG. La diluizione utilizzata è di 1:500. il segnale è ottimo. Poter provare un anticorpo prima di comprarlo è un'ottima politica, non si rischia di spendere fondi inutilmente.
Date published: 2018-05-21
Rated 2 out of 5 by from Ok, antibody Was not able to any specific bands when using at 1:200 dilution for our Cell line when probing via WB. .
Date published: 2018-04-13
Rated 5 out of 5 by from wonderful antibody! 1:200 diluted for wb of rat proein,the band is strong and special
Date published: 2018-04-10
Rated 3 out of 5 by from Ok antibody Was not able to get clear specific bands when using at 1:200 dilution for mouse tissue lysates when probing via WB. With further optimization or a different application, it may work better though.
Date published: 2018-03-23
Rated 4 out of 5 by from Good I got this as trial sample. It is working well and I happy to use it.
Date published: 2018-03-12
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