
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Superoxide Dismutase 1/SOD1 CRISPR/Cas9 KO Plasmid (m) | sc-423069 | 20 µg | $397.00 |
Sod1 encodes the cytosolic Cu/Zn superoxide dismutase SOD1, a primary antioxidant enzyme that converts superoxide anion into hydrogen peroxide and oxygen to limit oxidative damage. By shaping cellular redox homeostasis, SOD1 influences mitochondrial function, protein quality control, and stress-responsive signaling pathways including NRF2-mediated antioxidant programs. Perturbation of SOD1 activity alters susceptibility to oxidative stress, neuroinflammation, and proteotoxicity, and SOD1 dysfunction is widely used as a mechanistic entry point for studying neurodegeneration and related motor neuron phenotypes. In mouse systems, Sod1 serves as a tractable model to connect reactive oxygen species handling with metabolism, aging-associated tissue injury, and redox-sensitive transcriptional regulation.
Superoxide Dismutase 1/SOD1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sod1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sod1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sod1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Superoxide Dismutase 1/SOD1 protein expression.
This CRISPR knockout system enables efficient generation of Sod1-deficient cell models for investigation of Superoxide Dismutase 1/SOD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
CRISPRs +/- HDRs
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.