Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Superoxide Dismutase 1/SOD1 CRISPR/Cas9 KO Plasmid (m): sc-423069
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Superoxide Dismutase 1/SOD1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Superoxide Dismutase 1/SOD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Superoxide Dismutase 1/SOD1 Antibody (B-1): sc-271014
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Superoxide Dismutase 1/SOD1 CRISPR/Cas9 KO Plasmid (m) | sc-423069 | 20 µg | $397.00 |
Overview
Sod1 encodes the cytosolic Cu/Zn superoxide dismutase SOD1, a primary antioxidant enzyme that converts superoxide anion into hydrogen peroxide and oxygen to limit oxidative damage. By shaping cellular redox homeostasis, SOD1 influences mitochondrial function, protein quality control, and stress-responsive signaling pathways including NRF2-mediated antioxidant programs. Perturbation of SOD1 activity alters susceptibility to oxidative stress, neuroinflammation, and proteotoxicity, and SOD1 dysfunction is widely used as a mechanistic entry point for studying neurodegeneration and related motor neuron phenotypes. In mouse systems, Sod1 serves as a tractable model to connect reactive oxygen species handling with metabolism, aging-associated tissue injury, and redox-sensitive transcriptional regulation.
Superoxide Dismutase 1/SOD1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sod1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sod1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sod1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Superoxide Dismutase 1/SOD1 protein expression.
This CRISPR knockout system enables efficient generation of Sod1-deficient cell models for investigation of Superoxide Dismutase 1/SOD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sod1 exon(s) critical for Superoxide Dismutase 1/SOD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sod1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Superoxide Dismutase 1/SOD1 CRISPR/Cas9 KO Plasmid (m) and Superoxide Dismutase 1/SOD1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sod1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Superoxide Dismutase 1/SOD1 HDR Plasmid (m) and Superoxide Dismutase 1/SOD1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sod1 homology arms to support homology-directed repair at defined Sod1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.