SUMF2 Antibody (D-3): sc-393119
- SUMF2 Antibody (D-3) is a mouse monoclonal IgG1 κ SUMF2 antibody provided at 200 µg/ml
- raised against amino acids 1-110 mapping at the N-terminus of SUMF2 of human origin
- SUMF2 Antibody (D-3) is recommended for detection of SUMF2 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SUMF2 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SUMF2 Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with SUMF2 Antibody (D-3) (see ordering information below).
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SUMF2 Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects SUMF2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SUMF2 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SUMF2, also known as sulfatase-modifying factor 2, plays a crucial role in the activation of sulfatases, which are essential enzymes that hydrolyze sulfate esters to remove sulfate residues from diverse substrates. The proper functioning of sulfatases depends on the presence of Cα-formylglycine (FGly), a unique amino acid generated post-translationally by specific enzymes from cysteine residues. SUMF2 is primarily localized in the lumen of the endoplasmic reticulum and acts as an FGly-generating enzyme, although SUMF2 exhibits low catalytic activity when functioning alone. However, in a heterodimeric complex with SUMF1, another FGly-generating protein, SUMF2 significantly enhances catalytic efficiency. SUMF2 consists of 301 amino acids and is expressed in various human tissues, including the lung, heart, placenta, brain, liver, pancreas, skeletal muscle, and kidney, highlighting SUMF2′s importance in multiple physiological processes. Alternative splicing of the SUMF2 gene results in the production of four distinct isoforms, further contributing to the complexity of SUMF2′s functional roles in cellular metabolism and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SUMF2 Antibody (D-3) References:
- Multiple sulfatase deficiency is caused by mutations in the gene encoding the human C(alpha)-formylglycine generating enzyme. | Dierks, T., et al. 2003. Cell. 113: 435-44. PMID: 12757705
- The multiple sulfatase deficiency gene encodes an essential and limiting factor for the activity of sulfatases. | Cosma, MP., et al. 2003. Cell. 113: 445-56. PMID: 12757706
- The human SUMF1 gene, required for posttranslational sulfatase modification, defines a new gene family which is conserved from pro- to eukaryotes. | Landgrebe, J., et al. 2003. Gene. 316: 47-56. PMID: 14563551
- Crystal structure of human pFGE, the paralog of the Calpha-formylglycine-generating enzyme. | Dickmanns, A., et al. 2005. J Biol Chem. 280: 15180-7. PMID: 15687489
- Expression, localization, structural, and functional characterization of pFGE, the paralog of the Calpha-formylglycine-generating enzyme. | Mariappan, M., et al. 2005. J Biol Chem. 280: 15173-9. PMID: 15708861
- Sulphatase activities are regulated by the interaction of sulphatase-modifying factor 1 with SUMF2. | Zito, E., et al. 2005. EMBO Rep. 6: 655-60. PMID: 15962010
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SUMF2 Antibody (D-3) | sc-393119 | 200 µg/ml | $322.00 | |||
SUMF2 Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-530361 | 200 µg Ab; 10 µg BP | $361.00 | |||
SUMF2 Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-523813 | 200 µg Ab, 40 µg BP | $361.00 | |||
SUMF2 Antibody (D-3): m-IgG1 BP-HRP Bundle | sc-544013 | 200 µg Ab; 20 µg BP | $361.00 | |||
SUMF2 Antibody (D-3) AC | sc-393119 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SUMF2 Antibody (D-3) HRP | sc-393119 HRP | 200 µg/ml | $322.00 | |||
SUMF2 Antibody (D-3) FITC | sc-393119 FITC | 200 µg/ml | $336.00 | |||
SUMF2 Antibody (D-3) PE | sc-393119 PE | 200 µg/ml | $349.00 | |||
SUMF2 Antibody (D-3) Alexa Fluor® 488 | sc-393119 AF488 | 200 µg/ml | $364.00 | |||
SUMF2 Antibody (D-3) Alexa Fluor® 546 | sc-393119 AF546 | 200 µg/ml | $364.00 | |||
SUMF2 Antibody (D-3) Alexa Fluor® 594 | sc-393119 AF594 | 200 µg/ml | $364.00 | |||
SUMF2 Antibody (D-3) Alexa Fluor® 647 | sc-393119 AF647 | 200 µg/ml | $364.00 | |||
SUMF2 Antibody (D-3) Alexa Fluor® 680 | sc-393119 AF680 | 200 µg/ml | $364.00 | |||
SUMF2 Antibody (D-3) Alexa Fluor® 790 | sc-393119 AF790 | 200 µg/ml | $364.00 |