Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SULT1E1 CRISPR/Cas9 KO Plasmid (m): sc-423185
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SULT1E1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SULT1E1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SULT1E1 Antibody (E-12): sc-376009
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SULT1E1 CRISPR/Cas9 KO Plasmid (m) | sc-423185 | 20 µg | $397.00 |
Overview
Sult1e1 encodes estrogen sulfotransferase (SULT1E1), a cytosolic phase II conjugation enzyme that catalyzes sulfation of estrogens to generate inactive, more water-soluble steroid sulfates. By regulating the balance between active and conjugated estrogens, SULT1E1 influences estrogen receptor signaling and cross-talk with hepatic xenobiotic metabolism pathways, including interactions with steroid homeostasis, bile acid handling, and broader sulfotransferase networks. Altered SULT1E1 activity can shift hormone-dependent transcriptional programs and has been associated with metabolic and inflammatory states in liver and adipose tissues, as well as hormone-responsive phenotypes in reproductive biology. In mouse models, Sult1e1 is therefore relevant for studying endocrine regulation, sex differences in metabolism, and tissue-specific control of steroid signaling.
SULT1E1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sult1e1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sult1e1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sult1e1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SULT1E1 protein expression.
This CRISPR knockout system enables efficient generation of Sult1e1-deficient cell models for investigation of SULT1E1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sult1e1 exon(s) critical for SULT1E1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sult1e1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SULT1E1 CRISPR/Cas9 KO Plasmid (m) and SULT1E1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sult1e1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SULT1E1 HDR Plasmid (m) and SULT1E1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sult1e1 homology arms to support homology-directed repair at defined Sult1e1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.