Sulfiredoxin Antibody (E-2): sc-166747

Sulfiredoxin Antibody (E-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Sulfiredoxin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Sulfiredoxin antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sulfiredoxin, also known as Sulfiredoxin-1 or chromosome 20 open reading frame 139 (C20orf139), is a crucial cytoplasmic antioxidant that plays a significant role in cellular signaling by catalytically reducing oxidative modifications. Sulfiredoxin is essential for regulating peroxiredoxins (PRXs), a family of enzymes that mitigate oxidative stress by reducing hydroperoxides. Sulfiredoxin specifically targets conserved cysteine residues in PRDX1, PRDX2, PRDX3, and PRDX4, converting sulfinic acid back to sulfenic acid, which is vital for maintaining PRX functionality in various signaling pathways. Sulfiredoxin does not interact with PRDX5 or PRDX6, highlighting specificity. Sulfiredoxin is widely expressed in various tissues, with highest levels found in lung, spleen, kidney, and thymus, underscoring importance in antioxidant defense and cellular homeostasis.

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Sulfiredoxin Antibody (E-2) References:

1. Characterization of mammalian sulfiredoxin and its reactivation of hyperoxidized peroxiredoxin through reduction of cysteine sulfinic acid in the active site to cysteine.  |  Chang, TS., et al. 2004. J Biol Chem. 279: 50994-1001. PMID: 15448164
2. Reduction of cysteine sulfinic acid by sulfiredoxin is specific to 2-cys peroxiredoxins.  |  Woo, HA., et al. 2005. J Biol Chem. 280: 3125-8. PMID: 15590625
3. Oxidation of a eukaryotic 2-Cys peroxiredoxin is a molecular switch controlling the transcriptional response to increasing levels of hydrogen peroxide.  |  Bozonet, SM., et al. 2005. J Biol Chem. 280: 23319-27. PMID: 15824112
4. Evolution of eukaryotic cysteine sulfinic acid reductase, sulfiredoxin (Srx), from bacterial chromosome partitioning protein ParB.  |  Basu, MK. and Koonin, EV. 2005. Cell Cycle. 4: 947-52. PMID: 15917647
5. Structural basis for the retroreduction of inactivated peroxiredoxins by human sulfiredoxin.  |  Jönsson, TJ., et al. 2005. Biochemistry. 44: 8634-42. PMID: 15952770
6. Sulfiredoxin: a potential therapeutic agent?  |  Findlay, VJ., et al. 2005. Biomed Pharmacother. 59: 374-9. PMID: 16102934
7. 1H, 15N, and 13C chemical shift assignments of the human Sulfiredoxin (hSrx).  |  Lee, DY., et al. 2005. J Biomol NMR. 32: 339. PMID: 16211494
8. Sulfiredoxin, the cysteine sulfinic acid reductase specific to 2-Cys peroxiredoxin: its discovery, mechanism of action, and biological significance.  |  Rhee, SG., et al. 2007. Kidney Int Suppl. S3-8. PMID: 17653208
9. Reduction of cysteine sulfinic acid in eukaryotic, typical 2-Cys peroxiredoxins by sulfiredoxin.  |  Lowther, WT. and Haynes, AC. 2011. Antioxid Redox Signal. 15: 99-109. PMID: 20712415
10. The thioredoxin/peroxiredoxin/sulfiredoxin system: current overview on its redox function in plants and regulation by reactive oxygen and nitrogen species.  |  Sevilla, F., et al. 2015. J Exp Bot. 66: 2945-55. PMID: 25873657
11. Sulfiredoxin Promotes Cancer Cell Invasion through Regulation of the miR143-Fascin Axis.  |  Jiang, H., et al. 2022. Mol Cell Biol. 42: e0005122. PMID: 35412358
12. Comparative analysis of sulfiredoxin and total oxidative stress levels in diabetic individuals with periodontitis: A case-control study.  |  Tk, F., et al. 2023. J Periodontol. 94: 785-792. PMID: 36542371

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sulfiredoxin Antibody (E-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Sulfiredoxin Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sulfiredoxin Antibody (E-2) Specifications

Alternate Product Name: Sulfiredoxin

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 121-137

Epitope Target: Sulfiredoxin

Epitope Species: human

Product Reactivity/Detection: Sulfiredoxin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Sulfiredoxin Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin