Sulf-2 Antibody (G-4): sc-271772

Sulf-2 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sulf-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sulf-2 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sulf-2, also known as sulfatase 2 or HSULF-2, is an extracellular endosulfatase that plays a crucial role in regulating heparan sulfate proteoglycans (HSPGs) by hydrolyzing sulfate ester bonds. This enzymatic activity modulates HSPG interactions with various signaling molecules, essential for numerous biological processes, including cell growth, differentiation, and tumor progression. Sulf-2′s ability to interact with HSPGs is particularly important as these molecules are key components of cell surfaces and extracellular matrices, influencing growth factor activities and mediating cell adhesion. Additionally, HSPG regulation by Sulf-2 is implicated in tumor cell metastasis, highlighting Sulf-2′s significant role in cancer biology. Using anti-Sulf-2 antibody (G-4) in research can provide valuable insights into HSPG functional dynamics and their impact on cellular signaling pathways.

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Sulf-2 Antibody (G-4) References:

1. Cloning and characterization of two extracellular heparin-degrading endosulfatases in mice and humans.  |  Morimoto-Tomita, M., et al. 2002. J Biol Chem. 277: 49175-85. PMID: 12368295
2. Compositional profiling of heparin/heparan sulfate using mass spectrometry: assay for specificity of a novel extracellular human endosulfatase.  |  Saad, OM., et al. 2005. Glycobiology. 15: 818-26. PMID: 15843596
3. HSulf-1 and HSulf-2 are potent inhibitors of myeloma tumor growth in vivo.  |  Dai, Y., et al. 2005. J Biol Chem. 280: 40066-73. PMID: 16192265
4. Sulf-2, a proangiogenic heparan sulfate endosulfatase, is upregulated in breast cancer.  |  Morimoto-Tomita, M., et al. 2005. Neoplasia. 7: 1001-10. PMID: 16331886
5. HSulf-2, an extracellular endoglucosamine-6-sulfatase, selectively mobilizes heparin-bound growth factors and chemokines: effects on VEGF, FGF-1, and SDF-1.  |  Uchimura, K., et al. 2006. BMC Biochem. 7: 2. PMID: 16417632
6. The consensus coding sequences of human breast and colorectal cancers.  |  Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
7. Measuring the activities of the Sulfs: two novel heparin/heparan sulfate endosulfatases.  |  Uchimura, K., et al. 2006. Methods Enzymol. 416: 243-53. PMID: 17113870
8. Extracellular sulfatases, elements of the Wnt signaling pathway, positively regulate growth and tumorigenicity of human pancreatic cancer cells.  |  Nawroth, R., et al. 2007. PLoS One. 2: e392. PMID: 17460759
9. Human sulfatases: a structural perspective to catalysis.  |  Ghosh, D. 2007. Cell Mol Life Sci. 64: 2013-22. PMID: 17558559
10. Sulf-2: an extracellular modulator of cell signaling and a cancer target candidate.  |  Rosen, SD. and Lemjabbar-Alaoui, H. 2010. Expert Opin Ther Targets. 14: 935-49. PMID: 20629619
11. Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity.  |  El Masri, R., et al. 2022. Cell Rep. 38: 110516. PMID: 35294879
12. Galectin-3-Binding Protein Inhibits Extracellular Heparan 6-O-Endosulfatase Sulf-2.  |  Panigrahi, A., et al. 2024. Mol Cell Proteomics. 23: 100793. PMID: 38825040

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sulf-2 Antibody (G-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Sulf-2 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sulf-2 Antibody (G-4) Specifications

Alternate Product Name: Sulf-2

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 481-560

Epitope Target: Sulf-2

Epitope Species: human

Product Reactivity/Detection: Sulf-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Sulf-2 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin