SUGT1 Antibody (B-10): sc-398625
- SUGT1 Antibody (B-10) is a mouse monoclonal IgG1 κ SUGT1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 141-365 mapping at the C-terminus of SUGT1 of human origin
- SUGT1 Antibody (B-10) is recommended for detection of SUGT1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SUGT1 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SUGT1 Antibody (B-10) for WB applications. These reagents are now offered in bundles with SUGT1 Antibody (B-10) (see ordering information below).
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SUGT1 Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects SUGT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SUGT1 (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SUGT1, also known as Suppressor of G2 allele of Skp1 p19 homolog, is crucial for regulating cell cycle, particularly during G1/S and G2/M transitions, which are vital for proper cell division and function. SUGT1 is predominantly located in nucleus and cytoplasm, where SUGT1 plays a significant role in kinetochore assembly and acts as a co-chaperone for heat shock protein 90 (HSP90). SUGT1 contains several important structural features, including a CS domain, a SGS domain, a p23 domain, and three tetratricopeptide repeats (TPR), which are essential for interactions with other proteins, such as Skp1 p19 and CUL1, components of SCF (Skp1-Cullin-F-box) ubiquitin ligase complex. These interactions are critical for protein degradation processes, highlighting SUGT1′s importance in maintaining cellular homeostasis. An isoform known as SUGT1B exists due to alternative splicing, further emphasizing SUGT1′s complexity and regulatory potential in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SUGT1 Antibody (B-10) References:
- SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex. | Kitagawa, K., et al. 1999. Mol Cell. 4: 21-33. PMID: 10445024
- Identification of a novel splice variant: human SGT1B (SUGT1B). | Niikura, Y. and Kitagawa, K. 2003. DNA Seq. 14: 436-41. PMID: 15018354
- Sgt1 is required for human kinetochore assembly. | Steensgaard, P., et al. 2004. EMBO Rep. 5: 626-31. PMID: 15133482
- Molecular cloning and characterization of SGT1.2, a novel splice variant of Homo sapiens SGT1. | Zou, X., et al. 2004. DNA Seq. 15: 140-3. PMID: 15346769
- The expression and function of Sgt1 protein in eukaryotic cells. | Spiechowicz, M. and Filipek, A. 2005. Acta Neurobiol Exp (Wars). 65: 161-5. PMID: 15960300
- Haplotype loss of HLA class I antigen as an escape mechanism from immune attack in lung cancer. | So, T., et al. 2005. Cancer Res. 65: 5945-52. PMID: 15994973
- 17-AAG, an Hsp90 inhibitor, causes kinetochore defects: a novel mechanism by which 17-AAG inhibits cell proliferation. | Niikura, Y., et al. 2006. Oncogene. 25: 4133-46. PMID: 16501598
- Density of Sgt1-immunopositive neurons is decreased in the cerebral cortex of Alzheimer's disease brain. | Spiechowicz, M., et al. 2006. Neurochem Int. 49: 487-93. PMID: 16698122
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SUGT1 Antibody (B-10) | sc-398625 | 200 µg/ml | $316.00 | |||
SUGT1 Antibody (B-10): m-IgG Fc BP-HRP Bundle | sc-530877 | 200 µg Ab; 10 µg BP | $354.00 | |||
SUGT1 Antibody (B-10): m-IgGκ BP-HRP Bundle | sc-524428 | 200 µg Ab, 40 µg BP | $354.00 | |||
SUGT1 Antibody (B-10): m-IgG1 BP-HRP Bundle | sc-544293 | 200 µg Ab; 20 µg BP | $354.00 | |||
SUGT1 Antibody (B-10) AC | sc-398625 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SUGT1 Antibody (B-10) HRP | sc-398625 HRP | 200 µg/ml | $316.00 | |||
SUGT1 Antibody (B-10) FITC | sc-398625 FITC | 200 µg/ml | $330.00 | |||
SUGT1 Antibody (B-10) PE | sc-398625 PE | 200 µg/ml | $343.00 | |||
SUGT1 Antibody (B-10) Alexa Fluor® 488 | sc-398625 AF488 | 200 µg/ml | $357.00 | |||
SUGT1 Antibody (B-10) Alexa Fluor® 546 | sc-398625 AF546 | 200 µg/ml | $357.00 | |||
SUGT1 Antibody (B-10) Alexa Fluor® 594 | sc-398625 AF594 | 200 µg/ml | $357.00 | |||
SUGT1 Antibody (B-10) Alexa Fluor® 647 | sc-398625 AF647 | 200 µg/ml | $357.00 | |||
SUGT1 Antibody (B-10) Alexa Fluor® 680 | sc-398625 AF680 | 200 µg/ml | $357.00 | |||
SUGT1 Antibody (B-10) Alexa Fluor® 790 | sc-398625 AF790 | 200 µg/ml | $357.00 |