UBASH3B/STS1 Antibody (E-8): sc-514612
- UBASH3B/STS1 Antibody (E-8) is a mouse monoclonal IgG1 κ UBASH3B/STS1 antibody provided at 200 µg/ml
- raised against amino acids 101-208 mapping within an internal region of STS-1 of human origin
- UBASH3B/STS1 Antibody (E-8) is recommended for detection of STS-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-UBASH3B/STS1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for UBASH3B/STS1 Antibody (E-8) for WB applications. These reagents are now offered in bundles with UBASH3B/STS1 Antibody (E-8) (see ordering information below).
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STS-1 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects STS-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-STS-1 antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. STS-1, also known as UBASH3B or p70, plays a crucial role in negatively regulating signaling pathways downstream of the T cell receptor, which is vital for maintaining immune system balance and preventing overactivation of T cells. STS-1 is primarily localized in the cytoplasm and nucleus, with expression levels varying across different tissues; notably, STS-1 is minimally expressed in the pancreas, ovary, and heart. Structurally, STS-1 features an N-terminal ubiquitin-associated domain, a single SH3 domain, and a C-terminal domain that resembles the catalytic domain of phosphoglycerate mutases, which contributes to phosphatase activity. STS-1 interacts with activated epidermal growth factor receptor complexes, inhibiting their endocytosis and thereby regulating receptor internalization and cellular responses to growth factors. This regulatory function underscores STS-1 ′s significance in controlling receptor tyrosine kinase endocytosis and broader implications in cellular signaling and immune response modulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
UBASH3B/STS1 Antibody (E-8) References:
- Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins. | Nagase, T., et al. 2001. DNA Res. 8: 319-27. PMID: 11853319
- Identification, cDNA cloning, and targeted deletion of p70, a novel, ubiquitously expressed SH3 domain-containing protein. | Carpino, N., et al. 2002. Mol Cell Biol. 22: 7491-500. PMID: 12370296
- Regulation of ZAP-70 activation and TCR signaling by two related proteins, Sts-1 and Sts-2. | Carpino, N., et al. 2004. Immunity. 20: 37-46. PMID: 14738763
- Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases. | Kowanetz, K., et al. 2004. J Biol Chem. 279: 32786-95. PMID: 15159412
- Crystallization and initial crystal characterization of the C-terminal phosphoglycerate mutase homology domain of Sts-1. | Kleinman, H., et al. 2006. Acta Crystallogr Sect F Struct Biol Cryst Commun. 62: 218-20. PMID: 16511305
- An unsuspected ecdysteroid/steroid phosphatase activity in the key T-cell regulator, Sts-1: surprising relationship to insect ecdysteroid phosphate phosphatase. | Davies, L., et al. 2007. Proteins. 67: 720-31. PMID: 17348005
- A phosphatase activity of Sts-1 contributes to the suppression of TCR signaling. | Mikhailik, A., et al. 2007. Mol Cell. 27: 486-97. PMID: 17679096
- Suppressor of T-cell receptor signalling 1 and 2 differentially regulate endocytosis and signalling of receptor tyrosine kinases. | Raguz, J., et al. 2007. FEBS Lett. 581: 4767-72. PMID: 17880946
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBASH3B/STS1 Antibody (E-8) | sc-514612 | 200 µg/ml | $322.00 | |||
UBASH3B/STS1 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-531176 | 200 µg Ab; 10 µg BP | $361.00 | |||
UBASH3B/STS1 Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-524790 | 200 µg Ab, 40 µg BP | $361.00 | |||
UBASH3B/STS1 Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-544424 | 200 µg Ab; 20 µg BP | $361.00 | |||
UBASH3B/STS1 Antibody (E-8) AC | sc-514612 AC | 500 µg/ml, 25% agarose | $424.00 | |||
UBASH3B/STS1 Antibody (E-8) HRP | sc-514612 HRP | 200 µg/ml | $322.00 | |||
UBASH3B/STS1 Antibody (E-8) FITC | sc-514612 FITC | 200 µg/ml | $336.00 | |||
UBASH3B/STS1 Antibody (E-8) PE | sc-514612 PE | 200 µg/ml | $349.00 | |||
UBASH3B/STS1 Antibody (E-8) Alexa Fluor® 488 | sc-514612 AF488 | 200 µg/ml | $364.00 | |||
UBASH3B/STS1 Antibody (E-8) Alexa Fluor® 546 | sc-514612 AF546 | 200 µg/ml | $364.00 | |||
UBASH3B/STS1 Antibody (E-8) Alexa Fluor® 594 | sc-514612 AF594 | 200 µg/ml | $364.00 | |||
UBASH3B/STS1 Antibody (E-8) Alexa Fluor® 647 | sc-514612 AF647 | 200 µg/ml | $364.00 | |||
UBASH3B/STS1 Antibody (E-8) Alexa Fluor® 680 | sc-514612 AF680 | 200 µg/ml | $364.00 | |||
UBASH3B/STS1 Antibody (E-8) Alexa Fluor® 790 | sc-514612 AF790 | 200 µg/ml | $364.00 |