Streptavidin Antibody (S8C12): sc-52236
- Streptavidin Antibody (S8C12) is a mouse monoclonal IgG1 provided at 100 µg/ml
- raised against Streptavidin of Streptomyces avidinii origin
- recommended for detection of Streptavidin of Streptomyces avidinii origin by WB, IF and FCM; permits the formation of antibody-Streptavidin complexes, thus enhancing the sensitivity of the detection system
- available conjugated to FITC for IF, IHC(P) and FCM
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Streptavidin Antibody (S8C12). This work is in progress.
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Streptavidin Antibody (S8C12) is a mouse monoclonal IgG1 antibody that detects Streptavidin from Streptomyces avidinii through applications such as western blotting (WB), immunofluorescence (IF), and flow cytometry (FCM). Streptavidin is a tetrameric protein known for exceptional biotin-binding ability, showing one of the strongest known biological and noncovalent interactions. Each Streptavidin monomer binds a single biotin molecule, creating a robust Streptavidin-Biotin bond valuable in biochemical applications, including immunochemistry purification and diagnostic kit development. This interaction enables attachment of diverse chemicals to surfaces and facilitates molecule linkage, such as radioisotopes and monoclonal antibodies, enhancing assay sensitivity and specificity. Streptavidin′s versatility makes anti-Streptavidin antibody (S8C12) essential for researchers using Streptavidin-Biotin systems in experimental designs.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Streptavidin Antibody (S8C12) References:
- Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. | Lim, KH., et al. 2013. Biotechnol Bioeng. 110: 57-67. PMID: 22806584
- Target-activated streptavidin-biotin controlled binding probe. | Wu, YP., et al. 2018. Chem Sci. 9: 770-776. PMID: 29629147
- Protease-resistant streptavidin for interaction proteomics. | Rafiee, MR., et al. 2020. Mol Syst Biol. 16: e9370. PMID: 32400114
- Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates. | Udompholkul, P., et al. 2021. Molecules. 26: PMID: 34204178
- Application of HRP-streptavidin bionanoparticles for potentiometric biotin determination. | Büyüktiryaki, S., et al. 2022. Bioelectrochemistry. 144: 107993. PMID: 34823072
- Amplifying Intermolecular Events by Streptavidin-Induced Proximity. | Li, H., et al. 2022. J Am Chem Soc. 144: 11377-11385. PMID: 35715211
- Oligonucleotide-Blocked Streptavidin for Biotinylation Analysis. | Taylor, SK., et al. 2023. Bioconjug Chem. 34: 92-96. PMID: 36006852
- Epifluorescent single-molecule counting with Streptavidin-Phycoerythrin conjugates. | Schaub, JM., et al. 2022. Anal Biochem. 659: 114955. PMID: 36265689
- Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation. | Cookis, T., et al. 2023. J Vis Exp.. PMID: 38224121
- Genetic engineering of streptavidin, a versatile affinity tag. | Sano, T., et al. 1998. J Chromatogr B Biomed Sci Appl. 715: 85-91. PMID: 9792500
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Streptavidin Antibody (S8C12) | sc-52236 | 100 µg/ml | $322.00 | |||
Streptavidin Antibody (S8C12) FITC | sc-52236 FITC | 100 tests in 2 ml | $336.00 |