Stim1 Antibody (F-2): sc-393705
- Stim1 Antibody (F-2) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 33-54 within an N-terminal extracellular domain of Stim1 of human origin
- recommended for detection of Stim1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Stim1 Antibody (F-2) for WB applications. These reagents are now offered in bundles with Stim1 Antibody (F-2) (see ordering information below).
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Stim1 Antibody (F-2) is a mouse monoclonal IgG1 antibody that detects Stromal Interaction Molecule 1 (Stim1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Stim1 is a crucial cell surface transmembrane glycoprotein that plays a significant role in mediating calcium influx, particularly following the depletion of intracellular calcium stores. Located primarily in the endoplasmic reticulum, Stim1 functions as a calcium sensor due to its EF-hand domain, which undergoes substantial conformational changes in response to fluctuating calcium levels. When intracellular calcium levels drop, Stim1 translocates to the plasma membrane, where Stim1 activates the calcium release-activated calcium (CRAC) channel subunit, TMEM142A/Orai1, facilitating the influx of extracellular calcium. This process is vital for various cellular functions, including secretion and transcription, and highlights the importance of calcium signaling in maintaining cellular homeostasis. Additionally, Stim1′s interaction with Stim2, which acts as a potent inhibitor of Stim1-mediated store-operated calcium entry, underscores Stim1′s regulatory role in calcium dynamics. The implications of Stim1 extend beyond basic cellular functions, as Stim1 has been associated with tumor growth suppression and the interactions between stromal and hematopoietic cells, making Stim1 a significant target for research in cancer biology and cellular signaling pathways.
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Stim1 Antibody (F-2) References:
- TRPC channels as STIM1-regulated SOCs. | Yuan, JP., et al. 2009. Channels (Austin). 3: 221-5. PMID: 19574740
- Socking It to cardiac hypertrophy: STIM1-mediated Ca2+ entry in the cardiomyocyte. | Rosenberg, P. 2011. Circulation. 124: 766-8. PMID: 21844088
- Diseases caused by mutations in ORAI1 and STIM1. | Lacruz, RS. and Feske, S. 2015. Ann N Y Acad Sci. 1356: 45-79. PMID: 26469693
- The role of STIM1 and SOCE in smooth muscle contractility. | Feldman, CH., et al. 2017. Cell Calcium. 63: 60-65. PMID: 28372809
- miRNA-185 regulates retained fetal membranes of cattle by targeting STIM1. | Zheng, CY., et al. 2019. Theriogenology. 126: 166-171. PMID: 30553977
- STIM1 Regulates Endothelial Calcium Overload and Cytokine Upregulation During Sepsis. | Qiu, X., et al. 2021. J Surg Res. 263: 236-244. PMID: 33713955
- Relevance of stromal interaction molecule 1 (STIM1) in experimental and human stroke. | Stanzione, R., et al. 2022. Pflugers Arch. 474: 141-153. PMID: 34757454
- STIM1 and ORAI1 form a novel cold transduction mechanism in sensory and sympathetic neurons. | Buijs, TJ., et al. 2023. EMBO J. 42: e111348. PMID: 36524441
- Commentary on: Vanillic acid restores homeostasis of intestinal epithelium in colitis through inhibiting CA9/STIM1-mediated ferroptosis. | Xiong, Y., et al. 2024. Pharmacol Res. 204: 107226. PMID: 38777111
- STIM1 and lipid interactions at ER-PM contact sites. | Ke, Y., et al. 2025. Am J Physiol Cell Physiol. 328: C107-C114. PMID: 39620863
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stim1 Antibody (F-2) | sc-393705 | 200 µg/ml | $322.00 | |||
Stim1 Antibody (F-2): m-IgG Fc BP-HRP Bundle | sc-538287 | 200 µg Ab; 10 µg BP | $361.00 | |||
Stim1 Antibody (F-2): m-IgGκ BP-HRP Bundle | sc-535868 | 200 µg Ab; 40 µg BP | $361.00 | |||
Stim1 Antibody (F-2): m-IgG1 BP-HRP Bundle | sc-545623 | 200 µg Ab; 20 µg BP | $361.00 | |||
Stim1 (F-2) Neutralizing Peptide | sc-393705 P | 100 µg/0.5 ml | $69.00 |