Stim1 Antibody (A-8): sc-166840

Stim1 Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Stim1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Stim1 antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Stim1, also known as stromal interaction molecule 1, is a critical component in regulating calcium influx, which is vital for numerous cellular processes such as secretion, gene transcription, and muscle contraction. Located primarily in the endoplasmic reticulum, Stim1 acts as a calcium sensor through its EF-hand domain, enabling detection of drops in intracellular calcium levels. Upon sensing low calcium, Stim1 undergoes significant conformational changes and translocates to the plasma membrane, where Stim1 activates the calcium release-activated calcium (CRAC) channel subunit TMEM142A/Orai1, facilitating the influx of extracellular calcium. This mechanism is essential for maintaining cellular homeostasis and signaling, and dysregulation of Stim1 has been implicated in various pathologies, including tumor growth suppression and the modulation of stromal-hematopoietic cell interactions.

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Stim1 Antibody (A-8) References:

1. STIM1: a novel phosphoprotein located at the cell surface.  |  Manji, SS., et al. 2000. Biochim Biophys Acta. 1481: 147-55. PMID: 11004585
2. Stromal interaction molecule 1 (STIM1), a transmembrane protein with growth suppressor activity, contains an extracellular SAM domain modified by N-linked glycosylation.  |  Williams, RT., et al. 2002. Biochim Biophys Acta. 1596: 131-7. PMID: 11983428
3. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane.  |  Zhang, SL., et al. 2005. Nature. 437: 902-5. PMID: 16208375
4. STIM1 regulates Ca2+ entry via arachidonate-regulated Ca2+-selective (ARC) channels without store depletion or translocation to the plasma membrane.  |  Mignen, O., et al. 2007. J Physiol. 579: 703-15. PMID: 17158173
5. Essential role of the N-terminus of murine Orai1 in store-operated Ca2+ entry.  |  Takahashi, Y., et al. 2007. Biochem Biophys Res Commun. 356: 45-52. PMID: 17343823
6. TRPC1: the link between functionally distinct store-operated calcium channels.  |  Ambudkar, IS., et al. 2007. Cell Calcium. 42: 213-23. PMID: 17350680
7. A hexahistidine-Zn2+-dye label reveals STIM1 surface exposure.  |  Hauser, CT. and Tsien, RY. 2007. Proc Natl Acad Sci U S A. 104: 3693-7. PMID: 17360414
8. Orai proteins interact with TRPC channels and confer responsiveness to store depletion.  |  Liao, Y., et al. 2007. Proc Natl Acad Sci U S A. 104: 4682-7. PMID: 17360584
9. Live-cell imaging reveals sequential oligomerization and local plasma membrane targeting of stromal interaction molecule 1 after Ca2+ store depletion.  |  Liou, J., et al. 2007. Proc Natl Acad Sci U S A. 104: 9301-6. PMID: 17517596
10. TRPC1, Orai1, and STIM1 in SOCE: Friends in tight spaces.  |  Ambudkar, IS., et al. 2017. Cell Calcium. 63: 33-39. PMID: 28089266
11. STIM1 and Orai1 regulate Ca2+ microdomains for activation of transcription.  |  Schober, R., et al. 2019. Biochim Biophys Acta Mol Cell Res. 1866: 1079-1091. PMID: 30408546
12. Molecular Mechanism Analysis of STIM1 Thermal Sensation.  |  Liu, X., et al. 2023. Cells. 12: PMID: 37998348

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Stim1 Antibody (A-8) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-Stim1 Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Stim1 Antibody (A-8) Specifications

Alternate Product Name: Stromal interaction molecule 1

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 441-620

Epitope Target: Stim1

Epitope Species: human

Product Reactivity/Detection: Stim1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for Stim1 Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin