Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
STI1 CRISPR/Cas9 KO Plasmid (m): sc-423190
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- STI1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the STI1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: STI1 Antibody (D-6): sc-390203
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
STI1 CRISPR/Cas9 KO Plasmid (m) | sc-423190 | 20 µg | $397.00 |
Overview
Stip1 encodes stress-inducible phosphoprotein 1 (STI1, also known as HOP), a co-chaperone that bridges HSP70 and HSP90 to coordinate protein folding, maturation, and stabilization of signaling proteins. In mouse cells, STI1 supports proteostasis and contributes to cellular responses to heat shock, oxidative stress, and other proteotoxic insults, influencing protein quality control and turnover. By modulating chaperone client processing, STI1 can shape pathways governing receptor and kinase signaling, mitochondrial function, and cytoskeletal dynamics. Dysregulated chaperone networks involving STI1 are relevant to models of neurodegeneration, cancer biology, and inflammatory stress responses, where altered proteome stability and stress signaling are common features.
STI1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Stip1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stip1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stip1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish STI1 protein expression.
This CRISPR knockout system enables efficient generation of Stip1-deficient cell models for investigation of STI1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stip1 exon(s) critical for STI1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stip1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by STI1 CRISPR/Cas9 KO Plasmid (m) and STI1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stip1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by STI1 HDR Plasmid (m) and STI1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stip1 homology arms to support homology-directed repair at defined Stip1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.