STI1 Antibody (D-6): sc-390203

STI1 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects STI1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). STI1 Antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Stress-induced phosphoprotein 1 (STI1) plays a crucial role as a co-chaperone for heat shock proteins HSP70 and HSP90, particularly during cellular heat shock response, which is vital for maintaining protein homeostasis under stress conditions. STI1 is predominantly located in the cytoplasm and can also be found in the nucleus, where STI1 assists in proper folding and stabilization of client proteins, thereby preventing aggregation and misfolding that can lead to cellular dysfunction. STI1′s ability to exist as either a monomer or a dimer enhances functional versatility, allowing STI1 to effectively organize and facilitate interaction between HSP70 and HSP90, which is essential for proper functioning of these chaperones. By acting as a scaffold for client protein recruitment to the relaxed, ADP-bound conformation of HSP90, STI1 suppresses ATP turnover during the loading phase, ensuring that client proteins are correctly processed and folded, which is critical for cellular resilience and response to stress.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

STI1 Antibody (D-6) References:

1. The cochaperone murine stress-inducible protein 1: overexpression, purification, and characterization.  |  van der Spuy, J., et al. 2001. Protein Expr Purif. 21: 462-9. PMID: 11281722
2. Regulation of Hsp90 ATPase activity by the co-chaperone Cdc37p/p50cdc37.  |  Siligardi, G., et al. 2002. J Biol Chem. 277: 20151-9. PMID: 11916974
3. Stress-inducible protein 1 is a cell surface ligand for cellular prion that triggers neuroprotection.  |  Zanata, SM., et al. 2002. EMBO J. 21: 3307-16. PMID: 12093732
4. The Hsp90 co-chaperones Cdc37 and Sti1 interact physically and genetically.  |  Abbas-Terki, T., et al. 2002. Biol Chem. 383: 1335-42. PMID: 12437126
5. Sti1 is a non-competitive inhibitor of the Hsp90 ATPase. Binding prevents the N-terminal dimerization reaction during the atpase cycle.  |  Richter, K., et al. 2003. J Biol Chem. 278: 10328-33. PMID: 12525481
6. Sti1 is a novel activator of the Ssa proteins.  |  Wegele, H., et al. 2003. J Biol Chem. 278: 25970-6. PMID: 12716905
7. Sti1 and Cdc37 can stabilize Hsp90 in chaperone complexes with a protein kinase.  |  Lee, P., et al. 2004. Mol Biol Cell. 15: 1785-92. PMID: 14742721
8. PrP cooperates with STI1 to regulate SOD activity in PrP-deficient neuronal cell line.  |  Sakudo, A., et al. 2005. Biochem Biophys Res Commun. 328: 14-9. PMID: 15670743
9. Molecular cloning and expression of a transformation-sensitive human protein containing the TPR motif and sharing identity to the stress-inducible yeast protein STI1.  |  Honoré, B., et al. 1992. J Biol Chem. 267: 8485-91. PMID: 1569099
10. Definition of the minimal fragments of Sti1 required for dimerization, interaction with Hsp70 and Hsp90 and in vivo functions.  |  Flom, G., et al. 2007. Biochem J. 404: 159-67. PMID: 17300223
11. Hop/Sti1 phosphorylation inhibits its co-chaperone function.  |  Röhl, A., et al. 2015. EMBO Rep. 16: 240-9. PMID: 25504578
12. The cytosolic cochaperone Sti1 is relevant for mitochondrial biogenesis and morphology.  |  Hoseini, H., et al. 2016. FEBS J. 283: 3338-52. PMID: 27412066

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-STI1 Antibody (D-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-STI1 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

STI1 Antibody (D-6) Specifications

Alternate Product Name: STI1

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 203-453

Epitope Target: STI1

Epitope Species: human

Product Reactivity/Detection: STI1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for STI1 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin