Ste7 Antibody (B-12): sc-393269
- Ste7 Antibody (B-12) is a mouse monoclonal IgG1 κ Ste7 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-24 at the N-terminus of Ste7 of Saccharomyces cerevisiae origin
- Ste7 Antibody (B-12) is recommended for detection of Ste7 of y origin by WB, IP, IF and ELISA
- Anti-Ste7 Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ste7 Antibody (B-12) for WB applications. These reagents are now offered in bundles with Ste7 Antibody (B-12) (see ordering information below).
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Ste7 Antibody (B-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ste7 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Ste7 (B-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ste7 protein plays a crucial role in the mitogen-activated protein kinase (MAPK) signaling cascade, which is essential for cellular responses to external stimuli. Specifically, Ste7 functions as a MAP kinase kinase (MAPKK) that activates downstream MAP kinases Fus3 and Kss1, which are pivotal in regulating gene expression related to mating and cell cycle arrest in yeast. Proper functioning of this signaling pathway is vital for yeast′s ability to respond to pheromonal signals, ensuring successful mating and adaptation to environmental changes. Using anti-Ste7 antibody (B-12) in research can provide insights into the intricate mechanisms of signal transduction and physiological processes governed by the MAPK pathway in Saccharomyces cerevisiae.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ste7 Antibody (B-12) References:
- STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast. | Rhodes, N., et al. 1990. Genes Dev. 4: 1862-74. PMID: 2276621
- FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation. | Elion, EA., et al. 1990. Cell. 60: 649-64. PMID: 2406028
- STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes. | Errede, B. and Ammerer, G. 1989. Genes Dev. 3: 1349-61. PMID: 2558054
- The yeast STE12 protein binds to the DNA sequence mediating pheromone induction. | Dolan, JW., et al. 1989. Proc Natl Acad Sci U S A. 86: 5703-7. PMID: 2668945
- A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae. | Courchesne, WE., et al. 1989. Cell. 58: 1107-19. PMID: 2673544
- Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases. | Teague, MA., et al. 1986. Proc Natl Acad Sci U S A. 83: 7371-5. PMID: 3532111
- The yeast pheromone response pathway: new insights into signal transmission. | Ferguson, B., et al. 1994. Cell Mol Biol Res. 40: 223-8. PMID: 7874199
- FAR1 links the signal transduction pathway to the cell cycle machinery in yeast. | Peter, M., et al. 1993. Cell. 73: 747-60. PMID: 8500168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ste7 Antibody (B-12) | sc-393269 | 200 µg/ml | $322.00 | |||
Ste7 Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-530411 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ste7 Antibody (B-12): m-IgGκ BP-HRP Bundle | sc-523879 | 200 µg Ab, 40 µg BP | $361.00 | |||
Ste7 Antibody (B-12): m-IgG1 BP-HRP Bundle | sc-544039 | 200 µg Ab; 20 µg BP | $361.00 | |||
Ste7 Antibody (B-12) AC | sc-393269 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Ste7 Antibody (B-12) HRP | sc-393269 HRP | 200 µg/ml | $322.00 | |||
Ste7 Antibody (B-12) FITC | sc-393269 FITC | 200 µg/ml | $336.00 | |||
Ste7 Antibody (B-12) PE | sc-393269 PE | 200 µg/ml | $349.00 | |||
Ste7 Antibody (B-12) Alexa Fluor® 488 | sc-393269 AF488 | 200 µg/ml | $364.00 | |||
Ste7 Antibody (B-12) Alexa Fluor® 546 | sc-393269 AF546 | 200 µg/ml | $364.00 | |||
Ste7 Antibody (B-12) Alexa Fluor® 594 | sc-393269 AF594 | 200 µg/ml | $364.00 | |||
Ste7 Antibody (B-12) Alexa Fluor® 647 | sc-393269 AF647 | 200 µg/ml | $364.00 | |||
Ste7 Antibody (B-12) Alexa Fluor® 680 | sc-393269 AF680 | 200 µg/ml | $364.00 | |||
Ste7 Antibody (B-12) Alexa Fluor® 790 | sc-393269 AF790 | 200 µg/ml | $364.00 | |||
Ste7 (B-12) Neutralizing Peptide | sc-393269 P | 100 µg/0.5 ml | $69.00 |