Stat5b Antibody (G-2): sc-1656

Stat5b Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that specifically recognizes an epitope mapping between amino acids 750-779 at the C-terminus of Stat5b of mouse origin. Stat5b monoclonal antibody (G-2) detects Stat5b of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC) on paraffin-embedded sections, and enzyme-linked immunosorbent assay (ELISA), and is also reactive with additional species including equine, canine, bovine, and porcine. Stat5b, or Signal Transducer and Activator of Transcription 5b, and also called STAT5 and GHISID2, is a critical transcription factor that plays a key role in cytokine and growth factor signaling pathways. Stat5b becomes activated in response to stimuli such as IL-2, IL-4, CSF-1, and growth hormones, typically through receptor tyrosine kinases or G protein-coupled receptors. Stat5b undergoes phosphorylation, dimerization, and translocation to the nucleus to regulate gene expression, influencing T-cell receptor signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. The significance of Stat5b is underscored by its role as the major liver-expressed Stat5 form implicated in acute promyelocytic leukemias (APLL) through fusion with the retinoic acid receptor alpha gene, and studies in Stat5b null mice have shown severely impaired lymphoid development and differentiation, highlighting its importance in hematopoiesis and immune function. Stat5b monoclonal antibody (G-2) is available in non-conjugated form and multiple conjugated forms, including agarose, HRP, PE, FITC, and various Alexa Fluor® conjugates, providing versatility for diverse research applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Stat5b Antibody (G-2) References:

1. The role of Stat5a and Stat5b in signaling by IL-2 family cytokines.  |  Lin, JX. and Leonard, WJ. 2000. Oncogene. 19: 2566-76. PMID: 10851055
2. Stat5a/b contribute to interleukin 7-induced B-cell precursor expansion, but abl- and bcr/abl-induced transformation are independent of stat5.  |  Sexl, V., et al. 2000. Blood. 96: 2277-83. PMID: 10979977
3. Transcription factor STAT5A is a substrate of Bruton's tyrosine kinase in B cells.  |  Mahajan, S., et al. 2001. J Biol Chem. 276: 31216-28. PMID: 11413148
4. Serine phosphorylation of GH-activated signal transducer and activator of transcription 5a (STAT5a) and STAT5b: impact on STAT5 transcriptional activity.  |  Park, SH., et al. 2001. Mol Endocrinol. 15: 2157-71. PMID: 11731617
5. STAT5a activation mediates the epithelial to mesenchymal transition induced by oncogenic RhoA.  |  Benitah, SA., et al. 2003. Mol Biol Cell. 14: 40-53. PMID: 12529425
6. Specific inhibition of Stat5a/b promotes apoptosis of IL-2-responsive primary and tumor-derived lymphoid cells.  |  Behbod, F., et al. 2003. J Immunol. 171: 3919-27. PMID: 14530308
7. Loss of tolerance and autoimmunity affecting multiple organs in STAT5A/5B-deficient mice.  |  Snow, JW., et al. 2003. J Immunol. 171: 5042-50. PMID: 14607901
8. STAT5A interacts with and is phosphorylated upon activation of the mu-opioid receptor.  |  Mazarakou, G. and Georgoussi, Z. 2005. J Neurochem. 93: 918-31. PMID: 15857395
9. ERBB4/HER4 potentiates STAT5A transcriptional activity by regulating novel STAT5A serine phosphorylation events.  |  Clark, DE., et al. 2005. J Biol Chem. 280: 24175-80. PMID: 15863494
10. Stat5a/b are essential for normal lymphoid development and differentiation.  |  Yao, Z., et al. 2006. Proc Natl Acad Sci U S A. 103: 1000-5. PMID: 16418296
11. STAT5B deficiency: Impacts on human growth and immunity.  |  Hwa, V. 2016. Growth Horm IGF Res. 28: 16-20. PMID: 26703237
12. STAT5b is a key effector of NRG-1/ERBB4-mediated myocardial growth.  |  Vaparanta, K., et al. 2023. EMBO Rep. 24: e56689. PMID: 37009825

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Stat5b Antibody (G-2) has 141 citations in a variety of scientific publications.

Primary antibodies like Anti-Stat5b Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Stat5b Antibody (G-2) Specifications

Alternate Product Name: Stat5b

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 750-779

Epitope Target: Stat5b

Epitope Species: mouse

Product Reactivity/Detection: Stat5b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 141 citations in various scientific publications

Available Conjugates/Forms for Stat5b Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent