Stat2 CRISPR/Cas9 KO Plasmid (m2): sc-423175-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stat2 CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Stat2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Stat2 Antibody (B-3): sc-514193
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stat2 CRISPR/Cas9 KO Plasmid (m2) | sc-423175-KO-2 | 20 µg | $397.00 |
Overview
Signal transducer and activator of transcription 2 (Stat2) is a core transcription factor in type I and type III interferon signaling in mouse cells, forming the ISGF3 complex with STAT1 and IRF9 to drive expression of interferon-stimulated genes. Upon IFNAR/IFNLR engagement, JAK1 and TYK2 phosphorylate STAT2 to coordinate antiviral defense programs, antigen presentation, and broader innate immune transcriptional remodeling. Stat2 activity shapes crosstalk with NF-κB and MAPK-associated inflammatory pathways and influences cell-state decisions during infection and immune activation. Dysregulated STAT2-dependent signaling has been linked to altered susceptibility to viral challenge and immune-mediated pathology, making Stat2 a widely used genetic node for mechanistic studies of interferon responses.
Stat2 CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Stat2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stat2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stat2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Stat2 protein expression.
This CRISPR knockout system enables efficient generation of Stat2-deficient cell models for investigation of Stat2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stat2 exon(s) critical for Stat2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stat2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Stat2 CRISPR/Cas9 KO Plasmid (m) and Stat2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stat2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Stat2 HDR Plasmid (m) and Stat2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stat2 homology arms to support homology-directed repair at defined Stat2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.