Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
StAR CRISPR/Cas9 KO Plasmid (h): sc-400961
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- StAR CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the StAR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: StAR Antibody (D-2): sc-166821
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
StAR CRISPR/Cas9 KO Plasmid (h) | sc-400961 | 20 µg | $397.00 |
Overview
STAR encodes steroidogenic acute regulatory protein (StAR), a mitochondrial membrane-associated factor that mediates the rate-limiting transfer of cholesterol from the outer to the inner mitochondrial membrane to initiate steroid hormone biosynthesis. This process supports pregnenolone production by CYP11A1 and integrates with cAMP/PKA signaling, mitochondrial dynamics, and lipid trafficking in adrenal and gonadal steroidogenic cells. Disruption of StAR-dependent cholesterol transport perturbs steroidogenesis and alters downstream endocrine-regulated transcriptional programs and cellular stress responses. Variants or loss of STAR function are associated with congenital adrenal steroidogenic failure phenotypes, making StAR a key target for mechanistic studies of mitochondrial cholesterol handling and steroidogenic pathway control.
StAR CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STAR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STAR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STAR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish StAR protein expression.
This CRISPR knockout system enables efficient generation of STAR-deficient cell models for investigation of StAR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STAR exon(s) critical for StAR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STAR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by StAR CRISPR/Cas9 KO Plasmid (h) and StAR CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STAR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by StAR HDR Plasmid (h) and StAR HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STAR homology arms to support homology-directed repair at defined STAR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.