Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
STAMBP CRISPR/Cas9 KO Plasmid (h): sc-404726
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- STAMBP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the STAMBP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: STAMBP Antibody (H-4): sc-271641
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
STAMBP CRISPR/Cas9 KO Plasmid (h) | sc-404726 | 20 µg | $397.00 |
Overview
STAMBP encodes a JAMM-family deubiquitinating enzyme (AMSH) that binds ESCRT-0 components such as STAM and HGS to edit ubiquitin chains on endocytosed cargo. By regulating ubiquitin-dependent sorting, STAMBP helps control receptor downregulation, endosomal trafficking, and lysosomal degradation, thereby shaping signaling outputs from pathways including EGFR and other growth factor receptors. STAMBP activity contributes to proteostasis and cellular stress responses through modulation of ubiquitin chain topology and cargo fate decisions at early endosomes. Disruption of STAMBP has been linked to neurodevelopmental phenotypes and immune dysregulation, making it a useful target for studying endosome–lysosome pathway defects and ubiquitin signaling in human cells.
STAMBP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STAMBP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STAMBP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STAMBP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish STAMBP protein expression.
This CRISPR knockout system enables efficient generation of STAMBP-deficient cell models for investigation of STAMBP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STAMBP exon(s) critical for STAMBP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STAMBP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by STAMBP CRISPR/Cas9 KO Plasmid (h) and STAMBP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STAMBP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by STAMBP HDR Plasmid (h) and STAMBP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STAMBP homology arms to support homology-directed repair at defined STAMBP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.