STAMBP Antibody (H-4): sc-271641

STAMBP Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects STAMBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-STAMBP antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. STAMBP, also known as AMSH, is a 424 amino acid protein that belongs to the peptidase M67C family and is ubiquitously expressed in various tissues. STAMBP functions as a zinc metalloprotease that specifically cleaves ′Lys-63′-linked polyubiquitin chains, which is essential for regulating protein degradation and cellular signaling pathways. By opposing ubiquitin-dependent sorting of receptors to lysosomes, STAMBP plays a significant role in signal transduction related to cell growth and induction of Myc mediated by interleukin-2 and granulocyte-macrophage colony-stimulating factor. STAMBP enhances bone morphogenetic protein signaling by counteracting inhibitory effects of Smad6 and Smad7, making STAMBP a vital player in various cellular processes. STAMBP′s structure includes the JAMM motif, which is crucial for protease activity and is sensitive to inhibition by N-ethylmaleimide, highlighting the importance of structural integrity for functional activity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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STAMBP Antibody (H-4) References:

1. Promoting bone morphogenetic protein signaling through negative regulation of inhibitory Smads.  |  Itoh, F., et al. 2001. EMBO J. 20: 4132-42. PMID: 11483516
2. An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein.  |  Li, H. and Seth, A. 2004. Oncogene. 23: 1801-8. PMID: 14755250
3. AMSH is an endosome-associated ubiquitin isopeptidase.  |  McCullough, J., et al. 2004. J Cell Biol. 166: 487-92. PMID: 15314065
4. Activation of the endosome-associated ubiquitin isopeptidase AMSH by STAM, a component of the multivesicular body-sorting machinery.  |  McCullough, J., et al. 2006. Curr Biol. 16: 160-5. PMID: 16431367
5. Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes.  |  Nakamura, M., et al. 2006. Genes Cells. 11: 593-606. PMID: 16716190
6. Interaction of AMSH with ESCRT-III and deubiquitination of endosomal cargo.  |  Agromayor, M. and Martin-Serrano, J. 2006. J Biol Chem. 281: 23083-91. PMID: 16760479
7. AMSH regulates calcium-sensing receptor signaling through direct interactions.  |  Herrera-Vigenor, F., et al. 2006. Biochem Biophys Res Commun. 347: 924-30. PMID: 16854379
8. Targeting of AMSH to endosomes is required for epidermal growth factor receptor degradation.  |  Ma, YM., et al. 2007. J Biol Chem. 282: 9805-9812. PMID: 17261583
9. Structural basis for specific cleavage of Lys 63-linked polyubiquitin chains.  |  Sato, Y., et al. 2008. Nature. 455: 358-62. PMID: 18758443
10. STAM-binding protein regulates melanoma metastasis through SLUG stabilization.  |  Iwakami, Y., et al. 2018. Biochem Biophys Res Commun. 507: 484-488. PMID: 30454887
11. Structural and functional characterization of ubiquitin variant inhibitors for the JAMM-family deubiquitinases STAMBP and STAMBPL1.  |  Guo, Y., et al. 2021. J Biol Chem. 297: 101107. PMID: 34425109
12. Exploratory study of blood biomarkers in patients with post-stroke epilepsy.  |  Abraira, L., et al. 2024. Eur Stroke J. 9: 763-771. PMID: 38557165

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-STAMBP Antibody (H-4) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-STAMBP Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

STAMBP Antibody (H-4) Specifications

Alternate Product Name: STAMBP

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 131-270

Epitope Target: STAMBP

Epitope Species: mouse

Product Reactivity/Detection: STAMBP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for STAMBP Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin