Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SSSCA1 CRISPR/Cas9 KO Plasmid (h): sc-406804
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SSSCA1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SSSCA1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SSSCA1 Antibody (G-12): sc-515430
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSSCA1 CRISPR/Cas9 KO Plasmid (h) | sc-406804 | 20 µg | $397.00 |
Overview
SSSCA1 (Sjögren syndrome/scleroderma autoantigen 1) encodes a conserved nuclear protein originally identified as an autoantigen in systemic autoimmune disease. Although its molecular functions are still being clarified, available evidence links SSSCA1 to nuclear homeostasis, protein–protein interactions, and cellular stress-responsive processes that influence gene regulation and cell-cycle control. Altered expression or immune recognition of SSSCA1 has been reported in contexts relevant to autoimmunity and cancer biology, making it a useful target for dissecting mechanisms of aberrant nuclear regulation. Studying SSSCA1 supports pathway-level investigation of how nuclear proteins contribute to immune-associated pathology and dysregulated growth programs.
SSSCA1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SSSCA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SSSCA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SSSCA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SSSCA1 protein expression.
This CRISPR knockout system enables efficient generation of SSSCA1-deficient cell models for investigation of SSSCA1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SSSCA1 exon(s) critical for SSSCA1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SSSCA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SSSCA1 CRISPR/Cas9 KO Plasmid (h) and SSSCA1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SSSCA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SSSCA1 HDR Plasmid (h) and SSSCA1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SSSCA1 homology arms to support homology-directed repair at defined SSSCA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.