SSRP1 Antibody (D-7): sc-74536

SSRP1 Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects SSRP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SSRP1 antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SSRP1 plays a crucial role in regulating gene expression, particularly in chromatin structure and function. SSRP1 functions as a key component of the FACT complex, essential for transcription of chromatin templates. Through interactions with nucleosomes and histone H2A/H2B dimers, SSRP1 facilitates nucleosome disassembly during transcription, allowing RNA polymerase to access DNA. This process ensures efficient transcription of protein-coding genes in the tightly packed chromatin environment. SSRP1 also interacts with serum response factors, serving as a positive co-regulatory protein that enhances SRF-dependent gene expression, highlighting SSRP1′s significance in cellular processes.

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SSRP1 Antibody (D-7) References:

1. The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins.  |  Orphanides, G., et al. 1999. Nature. 400: 284-8. PMID: 10421373
2. DNA-interactions and nuclear localisation of the chromosomal HMG domain protein SSRP1 from maize.  |  Röttgers, K., et al. 2000. Plant J. 23: 395-405. PMID: 10929132
3. Interaction of FACT, SSRP1, and the high mobility group (HMG) domain of SSRP1 with DNA damaged by the anticancer drug cisplatin.  |  Yarnell, AT., et al. 2001. J Biol Chem. 276: 25736-41. PMID: 11344167
4. The high-mobility-group box protein SSRP1/T160 is essential for cell viability in day 3.5 mouse embryos.  |  Cao, S., et al. 2003. Mol Cell Biol. 23: 5301-7. PMID: 12861016
5. Chromatin as an essential part of the transcriptional mechanism.  |  Felsenfeld, G. 1992. Nature. 355: 219-24. PMID: 1731219
6. FACT Proteins, SUPT16H and SSRP1, Are Transcriptional Suppressors of HIV-1 and HTLV-1 That Facilitate Viral Latency.  |  Huang, H., et al. 2015. J Biol Chem. 290: 27297-27310. PMID: 26378236
7. Blockage of SSRP1/Ets-1/Pim-3 signalling enhances chemosensitivity of nasopharyngeal carcinoma to docetaxel in vitro.  |  Ai, J., et al. 2016. Biomed Pharmacother. 83: 1022-1031. PMID: 27525970
8. The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein.  |  Wittmeyer, J. and Formosa, T. 1997. Mol Cell Biol. 17: 4178-90. PMID: 9199353
9. FACT, a factor that facilitates transcript elongation through nucleosomes.  |  Orphanides, G., et al. 1998. Cell. 92: 105-16. PMID: 9489704
10. The HMG domain protein SSRP1/PREIIBF is involved in activation of the human embryonic beta-like globin gene.  |  Dyer, MA., et al. 1998. Mol Cell Biol. 18: 2617-28. PMID: 9566881
11. Factors regulating the transcriptional elongation activity of RNA polymerase II.  |  Shilatifard, A. 1998. FASEB J. 12: 1437-46. PMID: 9806752
12. Requirement of RSF and FACT for transcription of chromatin templates in vitro.  |  LeRoy, G., et al. 1998. Science. 282: 1900-4. PMID: 9836642

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SSRP1 Antibody (D-7) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-SSRP1 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SSRP1 Antibody (D-7) Specifications

Alternate Product Name: SSRP1

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: SSRP1

Epitope Species: human

Product Reactivity/Detection: SSRP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for SSRP1 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin