SRY Antibody (H-1): sc-374224

SRY Antibody (H-1) is a mouse monoclonal IgG2a antibody that detects SRY (sex-determining region Y protein) in humans through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRY plays a crucial role as a transcriptional activator necessary for male sex determination in mammals, functioning as a testis-determining factor (TDF) that initiates the development of testes from undifferentiated gonadal tissue. SRY contains a high mobility group (HMG) box domain, which is essential for DNA-binding activity, allowing SRY to interact with specific target genes that drive male differentiation. This interaction sets off a cascade of genetic events leading to the formation of male reproductive structures. DNA-binding activity of SRY is regulated by post-translational modifications, including phosphorylation by protein kinase A (PKA), which can influence function and stability. Mutations in SRY can lead to conditions such as 46,XY gonadal dysgenesis, where individuals with XY chromosomes develop as phenotypic females due to improper gonadal development. Anti-SRY antibody (H-1) is an invaluable tool for researchers studying sex determination and related disorders, providing insights into the molecular mechanisms underlying these critical biological processes.

The preferred SRY antibody is SRY Antibody (SRY-15): sc-69842.