SRY Antibody (SRY-15): sc-69842
- SRY Antibody (SRY-15) is a mouse monoclonal IgG1 κ SRY antibody, cited in 1 publications, provided at 200 µg/ml
- raised against recombinant partial length SRY of human origin
- SRY Antibody (SRY-15) is recommended for detection of SRY of human origin by WB, IP and IF
- Anti-SRY Antibody (SRY-15) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SRY (SRY-15): sc-69842.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SRY Antibody (SRY-15) for WB applications. These reagents are now offered in bundles with SRY Antibody (SRY-15) (see ordering information below).
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SRY Antibody (SRY-15) is a mouse monoclonal IgG1 kappa light chain antibody that detects SRY protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-SRY antibody (SRY-15) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SRY protein, also known as sex-determining region Y protein or testis-determining factor (TDF), plays a crucial role as a transcriptional activator in male sex determination in mammals. Located in the nucleus, SRY protein is essential for initiating testes formation from undifferentiated gonadal tissue, a process vital for male reproductive structure development. SRY protein′s DNA-binding ability is critical for function, as SRY activates expression of genes necessary for testis development. Notably, DNA-binding activity of SRY protein is regulated by post-translational modifications, including phosphorylation by protein kinase A (PKA), which can influence stability and function. Mutations in SRY gene have been linked to conditions such as 46,XY gonadal dysgenesis, where individuals with XY chromosomes develop as phenotypic females due to improper gonadal development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SRY Antibody (SRY-15) References:
- The C-terminal nuclear localization signal of the sex-determining region Y (SRY) high mobility group domain mediates nuclear import through importin beta 1. | Forwood, JK., et al. 2001. J Biol Chem. 276: 46575-82. PMID: 11535586
- Equilibrium binding assays reveal the elevated stoichiometry and salt dependence of the interaction between full-length human sex-determining region on the Y chromosome (SRY) and DNA. | Baud, S., et al. 2002. J Biol Chem. 277: 18404-10. PMID: 11877434
- Thimet oligopeptidase expression is differentially regulated in neuroendocrine and spermatid cell lines by transcription factor binding to SRY (sex-determining region Y), CAAT and CREB (cAMP-response-element-binding protein) promoter consensus sequences. | Morrison, LS. and Pierotti, AR. 2003. Biochem J. 376: 189-97. PMID: 12911328
- The biochemical role of SRY in sex determination. | Harley, VR. and Goodfellow, PN. 1994. Mol Reprod Dev. 39: 184-93. PMID: 7826621
- The human SRY transcript. | Clépet, C., et al. 1993. Hum Mol Genet. 2: 2007-12. PMID: 8111368
- The role of SRY in mammalian sex determination. | Fechner, PY. 1996. Acta Paediatr Jpn. 38: 380-9. PMID: 8840551
- Analysis of the testis-determining gene SRY in patients with XY gonadal dysgenesis. | Tsutsumi, O., et al. 1996. Horm Res. 46 Suppl 1: 6-10. PMID: 8864741
- Phosphorylation of an N-terminal motif enhances DNA-binding activity of the human SRY protein. | Desclozeaux, M., et al. 1998. J Biol Chem. 273: 7988-95. PMID: 9525897
- Evolution of the mammalian Y chromosome and sex-determining genes. | Graves, JA. 1998. J Exp Zool. 281: 472-81. PMID: 9662834
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRY Antibody (SRY-15) | sc-69842 | 200 µg/ml | $316.00 | |||
SRY Antibody (SRY-15): m-IgG Fc BP-HRP Bundle | sc-528649 | 200 µg Ab; 10 µg BP | $354.00 | |||
SRY Antibody (SRY-15): m-IgGκ BP-HRP Bundle | sc-521086 | 200 µg Ab, 40 µg BP | $354.00 | |||
SRY Antibody (SRY-15) AC | sc-69842 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SRY Antibody (SRY-15) HRP | sc-69842 HRP | 200 µg/ml | $316.00 | |||
SRY Antibody (SRY-15) FITC | sc-69842 FITC | 200 µg/ml | $330.00 | |||
SRY Antibody (SRY-15) PE | sc-69842 PE | 200 µg/ml | $343.00 | |||
SRY Antibody (SRY-15) Alexa Fluor® 488 | sc-69842 AF488 | 200 µg/ml | $357.00 | |||
SRY Antibody (SRY-15) Alexa Fluor® 546 | sc-69842 AF546 | 200 µg/ml | $357.00 | |||
SRY Antibody (SRY-15) Alexa Fluor® 594 | sc-69842 AF594 | 200 µg/ml | $357.00 | |||
SRY Antibody (SRY-15) Alexa Fluor® 647 | sc-69842 AF647 | 200 µg/ml | $357.00 | |||
SRY Antibody (SRY-15) Alexa Fluor® 680 | sc-69842 AF680 | 200 µg/ml | $357.00 | |||
SRY Antibody (SRY-15) Alexa Fluor® 790 | sc-69842 AF790 | 200 µg/ml | $357.00 |