SRPK1 Antibody (EE-13): sc-100443

SRPK1 Antibody (EE-13) is a mouse monoclonal IgG1 kappa light chain antibody that detects SRPK1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-SRPK1 antibody (EE-13) is available as a non-conjugated format. SRPK1 plays a crucial role in the regulation of pre-mRNA splicing, a vital process that ensures correct expression of genes by removing introns and joining exons in mRNA transcripts. This regulation is essential for maintaining cellular function and adaptability, as it allows for production of multiple protein isoforms from a single gene, thereby increasing proteome diversity. SRPK1 specifically phosphorylates arginine/serine-rich (RS) domain-containing proteins, which are integral to splicing machinery, and SRPK1 activity is tightly regulated to ensure proper splicing occurs in coordination with transcription. Notably, SRPK1 is predominantly expressed in pancreas, highlighting SRPK1′s potential role in pancreatic function and development. Anti-SRPK1 antibody (EE-13)′s ability to detect SRPK1 across various applications makes this reagent an invaluable tool for researchers studying gene expression and splicing mechanisms in human cells.

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SRPK1 Antibody (EE-13) References:

1. Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes.  |  Wang, HY., et al. 1999. Genomics. 57: 310-5. PMID: 10198174
2. SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1.  |  Papoutsopoulou, S., et al. 1999. Nucleic Acids Res. 27: 2972-80. PMID: 10390541
3. Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1.  |  Tang, Z., et al. 2000. Mol Cell Biol. 20: 816-24. PMID: 10629038
4. Synthesis of selective SRPK-1 inhibitors: novel tricyclic quinoxaline derivatives.  |  Székelyhidi, Z., et al. 2005. Bioorg Med Chem Lett. 15: 3241-6. PMID: 15925511
5. Topical antiangiogenic SRPK1 inhibitors reduce choroidal neovascularization in rodent models of exudative AMD.  |  Gammons, MV., et al. 2013. Invest Ophthalmol Vis Sci. 54: 6052-62. PMID: 23887803
6. A theoretical study of SRPK interaction with the flexible domains of hepatitis B capsids.  |  Kim, J. and Wu, J. 2014. Biophys J. 107: 1453-61. PMID: 25229152
7. Potential Antileukemia Effect and Structural Analyses of SRPK Inhibition by N-(2-(Piperidin-1-yl)-5-(Trifluoromethyl)Phenyl)Isonicotinamide (SRPIN340).  |  Siqueira, RP., et al. 2015. PLoS One. 10: e0134882. PMID: 26244849
8. Antimetastatic effect of the pharmacological inhibition of serine/arginine-rich protein kinases (SRPK) in murine melanoma.  |  Moreira, GA., et al. 2018. Toxicol Appl Pharmacol. 356: 214-223. PMID: 30138656
9. The SRPK inhibitor N-(2-(piperidin-1-yl)-5-(trifluoromethyl)phenyl) isonicotinamide (SRPIN340) increases the immune response against metastatic melanoma in mice.  |  Moreira, GA., et al. 2022. Biochem Pharmacol. 203: 115161. PMID: 35787994
10. SRPK Inhibitors Reduce the Phosphorylation and Translocation of SR Protein Splicing Factors, thereby Correcting BIN1, MCL-1 and BCL2 Splicing Errors and Enabling Apoptosis of Cholangiocarcinoma Cells.  |  Changphasuk, P., et al. 2024. Front Biosci (Schol Ed). 16: 17. PMID: 39344395
11. Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles.  |  Kuroyanagi, N., et al. 1998. Biochem Biophys Res Commun. 242: 357-64. PMID: 9446799
12. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells.  |  Wang, HY., et al. 1998. J Cell Biol. 140: 737-50. PMID: 9472028

The preferred SRPK antibody is SRPK Antibody (D-7): sc-398432.