SRPK Antibody (D-7): sc-398432

SRPK Antibody (D-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects SRPK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SRPK antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. SRPK protein plays a crucial role in regulating pre-mRNA splicing, a vital process for gene expression, by phosphorylating arginine/serine-rich (RS) domain-containing proteins. This phosphorylation is essential for proper functioning of splicing factors, which coordinate splicing with transcription, ensuring accurate mRNA processing before translation. SRPK1, one of the key isoforms, is specifically involved in sperm chromatin condensation, highlighting its importance in reproductive biology. SRPK2 exhibits a strong preference for SR dipeptides and features a proline-rich sequence at its amino terminus, while SRPK3 is expressed in cardiac and skeletal muscle tissues. Differential expression of these kinases in various tissues underscores their specialized functions and intricate regulatory mechanisms governing splicing in different cellular contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SRPK Antibody (D-7) References:

1. Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes.  |  Wang, HY., et al. 1999. Genomics. 57: 310-5. PMID: 10198174
2. SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1.  |  Papoutsopoulou, S., et al. 1999. Nucleic Acids Res. 27: 2972-80. PMID: 10390541
3. Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1.  |  Tang, Z., et al. 2000. Mol Cell Biol. 20: 816-24. PMID: 10629038
4. Centronuclear myopathy in mice lacking a novel muscle-specific protein kinase transcriptionally regulated by MEF2.  |  Nakagawa, O., et al. 2005. Genes Dev. 19: 2066-77. PMID: 16140986
5. A kinase chaperones hepatitis B virus capsid assembly and captures capsid dynamics in vitro.  |  Chen, C., et al. 2011. PLoS Pathog. 7: e1002388. PMID: 22114561
6. The conserved kinase SRPK regulates karyosome formation and spindle microtubule assembly in Drosophila oocytes.  |  Loh, BJ., et al. 2012. J Cell Sci. 125: 4457-62. PMID: 22854045
7. Topical antiangiogenic SRPK1 inhibitors reduce choroidal neovascularization in rodent models of exudative AMD.  |  Gammons, MV., et al. 2013. Invest Ophthalmol Vis Sci. 54: 6052-62. PMID: 23887803
8. Recruiting a silent partner for activation of the protein kinase SRPK1.  |  Aubol, BE. and Adams, JA. 2014. Biochemistry. 53: 4625-34. PMID: 24984036
9. The SRPK inhibitor N-(2-(piperidin-1-yl)-5-(trifluoromethyl)phenyl) isonicotinamide (SRPIN340) increases the immune response against metastatic melanoma in mice.  |  Moreira, GA., et al. 2022. Biochem Pharmacol. 203: 115161. PMID: 35787994
10. SRPK Inhibitors Reduce the Phosphorylation and Translocation of SR Protein Splicing Factors, thereby Correcting BIN1, MCL-1 and BCL2 Splicing Errors and Enabling Apoptosis of Cholangiocarcinoma Cells.  |  Changphasuk, P., et al. 2024. Front Biosci (Schol Ed). 16: 17. PMID: 39344395
11. Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles.  |  Kuroyanagi, N., et al. 1998. Biochem Biophys Res Commun. 242: 357-64. PMID: 9446799
12. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells.  |  Wang, HY., et al. 1998. J Cell Biol. 140: 737-50. PMID: 9472028

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SRPK Antibody (D-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SRPK Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SRPK Antibody (D-7) Specifications

Alternate Product Name: SRPK

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 530-547

Epitope Target: SRPK1

Epitope Species: human

Product Reactivity/Detection: SRPK1, SRPK2 and SRPK3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SRPK Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin