SRp55 Antibody (C-6): sc-515111
- SRp55 Antibody (C-6) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 16-34 within RNA binding domain 1 near the N-terminus of SRp55 of human origin
- recommended for detection of SRp55 of mouse, rat and human origin by WB, IP, IF and ELISA
- See SR (1H4): sc-13509 for SRp55 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for SRp55 Antibody (C-6) for WB applications. This reagent is now offered in a bundle with SRp55 Antibody (C-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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SRp55 Antibody (C-6) is a mouse monoclonal IgM antibody that detects SRp55 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRp55, a member of the serine/arginine-rich (SR) protein family, plays a pivotal role in the regulation of pre-mRNA splicing, which is essential for proper gene expression. SRp55 is primarily located in the nucleus, where SRp55 participates in the assembly of spliceosomal complexes that facilitate intron removal from pre-mRNA. Nuclear localization of SRp55 enables timely and accurate mRNA processing, ensuring only mature mRNA reaches the cytoplasm for translation. SRp55 interacts with other splicing factors, such as SRp30c and htra2beta1, to regulate specific exon splicing, including FGFR1 alpha-exon inclusion, which is vital for normal cellular function and development. SRp55′s ability to modulate splicing decisions highlights SRp55′s importance in gene expression regulation and potential implications in various diseases when splicing is disrupted.
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SRp55 Antibody (C-6) References:
- SF2 and SRp55 regulation of CD45 exon 4 skipping during T cell activation. | Lemaire, R., et al. 1999. Eur J Immunol. 29: 823-37. PMID: 10092085
- Differential effects of hyperphosphorylation on splicing factor SRp55. | Lai, MC., et al. 2003. Biochem J. 371: 937-45. PMID: 12549978
- Enhancer-dependent splicing of FGFR1 alpha-exon is repressed by RNA interference-mediated down-regulation of SRp55. | Jin, W. and Cote, GJ. 2004. Cancer Res. 64: 8901-5. PMID: 15604250
- SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells. | Tardos, JG., et al. 2008. J Thromb Haemost. 6: 877-84. PMID: 18315555
- Splicing and splice factor SRp55 participate in the response to DNA damage by changing isoform ratios of target genes. | Filippov, V., et al. 2008. Gene. 420: 34-41. PMID: 18571879
- SRp40 and SRp55 promote the translation of unspliced human immunodeficiency virus type 1 RNA. | Swanson, CM., et al. 2010. J Virol. 84: 6748-59. PMID: 20427542
- Serine- and arginine-rich proteins 55 and 75 (SRp55 and SRp75) induce production of HIV-1 vpr mRNA by inhibiting the 5'-splice site of exon 3. | Tranell, A., et al. 2010. J Biol Chem. 285: 31537-47. PMID: 20685659
- Evidence for upregulation of Bim and the splicing factor SRp55 in melanoma cells from patients treated with selective BRAF inhibitors. | Lai, F., et al. 2012. Melanoma Res. 22: 244-51. PMID: 22516966
- Dual-specificity tyrosine phosphorylation-regulated kinase 1A (Dyrk1A) modulates serine/arginine-rich protein 55 (SRp55)-promoted Tau exon 10 inclusion. | Yin, X., et al. 2012. J Biol Chem. 287: 30497-506. PMID: 22767602
- Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55. | Nagel, RJ., et al. 1998. RNA. 4: 11-23. PMID: 9436904
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRp55 Antibody (C-6) | sc-515111 | 200 µg/ml | $322.00 | |||
SRp55 Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-536413 | 200 µg Ab; 40 µg BP | $361.00 | |||
SRp55 (C-6) Neutralizing Peptide | sc-515111 P | 100 µg/0.5 ml | $69.00 |