SRp55 Antibody (16H3): sc-57954

SRp55 Antibody (16H3) is a mouse monoclonal IgG1 kappa light chain antibody that detects SRp55 protein of mouse, rat, human, and Drosophila melanogaster origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-SRp55 antibody (16H3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SRp55, a member of the serine/arginine-rich (SR) protein family, plays a crucial role in pre-mRNA splicing, which is essential for post-transcriptional regulation of gene expression. Located predominantly in the nucleus, SRp55 is involved in spliceosomal complex assembly and alternative splicing event regulation. SRp55′s interaction with other splicing factors, such as SRp30c and htra2beta1, highlights SRp55′s importance in modulating exon inclusion and exclusion, particularly in fibroblast growth factor receptor 1 (FGFR1) alpha-exon inclusion. Precise regulation of splicing by SRp55 remains vital for maintaining proper gene expression and cellular function, making SRp55 a significant target for research in gene regulation and splicing mechanisms.

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SRp55 Antibody (16H3) References:

1. SF2 and SRp55 regulation of CD45 exon 4 skipping during T cell activation.  |  Lemaire, R., et al. 1999. Eur J Immunol. 29: 823-37. PMID: 10092085
2. Human transformer 2beta and SRp55 interact with a calcitonin-specific splice enhancer.  |  Tran, Q., et al. 2003. Biochim Biophys Acta. 1625: 141-52. PMID: 12531473
3. SRp55 is a regulator of calcitonin/CGRP alternative RNA splicing.  |  Tran, Q. and Roesser, JR. 2003. Biochemistry. 42: 951-7. PMID: 12549914
4. Differential effects of hyperphosphorylation on splicing factor SRp55.  |  Lai, MC., et al. 2003. Biochem J. 371: 937-45. PMID: 12549978
5. A minimal length between tau exon 10 and 11 is required for correct splicing of exon 10.  |  Yu, Q., et al. 2004. J Neurochem. 90: 164-72. PMID: 15198676
6. Enhancer-dependent splicing of FGFR1 alpha-exon is repressed by RNA interference-mediated down-regulation of SRp55.  |  Jin, W. and Cote, GJ. 2004. Cancer Res. 64: 8901-5. PMID: 15604250
7. Tau exons 2 and 10, which are misregulated in neurodegenerative diseases, are partly regulated by silencers which bind a SRp30c.SRp55 complex that either recruits or antagonizes htra2beta1.  |  Wang, Y., et al. 2005. J Biol Chem. 280: 14230-9. PMID: 15695522
8. Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm.  |  Tranell, A., et al. 2011. Virus Res. 157: 82-91. PMID: 21345357
9. SRp55 Regulates a Splicing Network That Controls Human Pancreatic β-Cell Function and Survival.  |  Juan-Mateu, J., et al. 2018. Diabetes. 67: 423-436. PMID: 29246973
10. A candidate gene study reveals association between a variant of the SRp55 splicing factor gene and systemic sclerosis.  |  Romano, E., et al. 2022. Clin Exp Rheumatol. 40: 1921-1925. PMID: 34665708
11. The SR protein B52/SRp55 is essential for Drosophila development.  |  Ring, HZ. and Lis, JT. 1994. Mol Cell Biol. 14: 7499-506. PMID: 7935465
12. Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55.  |  Nagel, RJ., et al. 1998. RNA. 4: 11-23. PMID: 9436904

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SRp55 Antibody (16H3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-SRp55 Antibody (16H3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SRp55 Antibody (16H3) Specifications

Alternate Product Name: SRSF6

Product Clone Name: 16H3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: SRp55

Epitope Species: bovine and Drosophila

Product Reactivity/Detection: SRp20, SRp40, SRp55, SRp75

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: Drosophila

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for SRp55 Antibody (16H3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SR antibody is SR Antibody (1H4): sc-13509.