SRP54 Antibody (H-8): sc-393855
- SRP54 Antibody (H-8) is a mouse monoclonal IgG1 κ SRP54 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 259-504 mapping at the C-terminus of SRP54 of human origin
- SRP54 Antibody (H-8) is recommended for detection of SRP54 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-SRP54 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SRP54 Antibody (H-8) for WB applications. These reagents are now offered in bundles with SRP54 Antibody (H-8) (see ordering information below).
QUICK LINKS
SRP54 Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects SRP54 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-SRP54 antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SRP54 plays a crucial role in the signal recognition particle (SRP) complex, which is essential for targeting nascent proteins to the endoplasmic reticulum (ER) during translation. This process is vital for proper localization and processing of membrane and secreted proteins, ensuring they reach functional destinations within the cell. SRP54 interacts with both the nascent signal peptide and SRP RNA, facilitating recognition and translocation of these proteins. Additionally, SRP54′s interaction with SRP RNA is critical for assembly of the SRP complex, which includes other proteins such as SRP19, SRP68, and SRP72. Localization of SRP54 in the cytoplasm, along with nucleolar and cytoplasmic presence of other SRP proteins, underscores intricate coordination required for effective protein synthesis and targeting. Using SRP54 (H-8) monoclonal antibody in research can provide valuable insights into mechanisms of protein translocation and SRP′s role in cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SRP54 Antibody (H-8) References:
- Signal recognition particle components in the nucleolus. | Politz, JC., et al. 2000. Proc Natl Acad Sci U S A. 97: 55-60. PMID: 10618370
- The nucleolus and the four ribonucleoproteins of translation. | Pederson, T. and Politz, JC. 2000. J Cell Biol. 148: 1091-5. PMID: 10725320
- Crystal structure of an early protein-RNA assembly complex of the signal recognition particle. | Wild, K., et al. 2001. Science. 294: 598-601. PMID: 11641499
- Systematic site-directed mutagenesis of human protein SRP54: interactions with signal recognition particle RNA and modes of signal peptide recognition. | Huang, Q., et al. 2002. Biochemistry. 41: 11362-71. PMID: 12234178
- RNA interference of signal peptide-binding protein SRP54 elicits deleterious effects and protein sorting defects in trypanosomes. | Liu, L., et al. 2002. J Biol Chem. 277: 47348-57. PMID: 12244113
- Isolation and characterization of a cDNA clone encoding the 19 kDa protein of signal recognition particle (SRP): expression and binding to 7SL RNA. | Lingelbach, K., et al. 1988. Nucleic Acids Res. 16: 9431-42. PMID: 2460823
- Cancer and immune-mediated necrotizing myopathy: a longitudinal referral case-controlled outcomes evaluation. | Shelly, S., et al. 2022. Rheumatology (Oxford). 62: 281-289. PMID: 35285492
- Acute myeloid leukemia in SRP54-mutated congenital neutropenia. | Sabulski, A., et al. 2022. EJHaem. 3: 521-525. PMID: 35846055
- Severe congenital neutropenia, SRP54 pathogenicity, and a framework for surveillance. | Fan, EM., et al. 2023. Am J Med Genet A. 191: 1434-1441. PMID: 36815775
- Subcellular distribution of signal recognition particle and 7SL-RNA determined with polypeptide-specific antibodies and complementary DNA probe. | Walter, P. and Blobel, G. 1983. J Cell Biol. 97: 1693-9. PMID: 6196367
- The uRNA database. | Zwieb, C. 1997. Nucleic Acids Res. 25: 102-3. PMID: 9016512
- Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites. | Gowda, K., et al. 1998. Gene. 207: 197-207. PMID: 9511762
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRP54 Antibody (H-8) | sc-393855 | 200 µg/ml | $316.00 | |||
SRP54 Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-530606 | 200 µg Ab; 10 µg BP | $354.00 | |||
SRP54 Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-524120 | 200 µg Ab, 40 µg BP | $354.00 | |||
SRP54 Antibody (H-8): m-IgG1 BP-HRP Bundle | sc-544160 | 200 µg Ab; 20 µg BP | $354.00 | |||
SRP54 Antibody (H-8) AC | sc-393855 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SRP54 Antibody (H-8) HRP | sc-393855 HRP | 200 µg/ml | $316.00 | |||
SRP54 Antibody (H-8) FITC | sc-393855 FITC | 200 µg/ml | $330.00 | |||
SRP54 Antibody (H-8) PE | sc-393855 PE | 200 µg/ml | $343.00 | |||
SRP54 Antibody (H-8) Alexa Fluor® 488 | sc-393855 AF488 | 200 µg/ml | $357.00 | |||
SRP54 Antibody (H-8) Alexa Fluor® 546 | sc-393855 AF546 | 200 µg/ml | $357.00 | |||
SRP54 Antibody (H-8) Alexa Fluor® 594 | sc-393855 AF594 | 200 µg/ml | $357.00 | |||
SRP54 Antibody (H-8) Alexa Fluor® 647 | sc-393855 AF647 | 200 µg/ml | $357.00 | |||
SRP54 Antibody (H-8) Alexa Fluor® 680 | sc-393855 AF680 | 200 µg/ml | $357.00 | |||
SRP54 Antibody (H-8) Alexa Fluor® 790 | sc-393855 AF790 | 200 µg/ml | $357.00 |