Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SRp30c CRISPR/Cas9 KO Plasmid (h): sc-403790
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SRp30c CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SRp30c genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SRp30c Antibody (1G7): sc-293314
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRp30c CRISPR/Cas9 KO Plasmid (h) | sc-403790 | 20 µg | $397.00 |
Overview
SRSF9 encodes the serine/arginine-rich splicing factor SRp30c, an RNA-binding protein that contributes to splice-site selection and exon definition during pre-mRNA processing. Through interactions with exonic splicing enhancers and other SR proteins, SRp30c helps coordinate alternative splicing programs that shape transcript isoform output, coupling RNA processing to transcription and downstream mRNA fate. These splicing decisions can influence cell-cycle control, stress responses, and differentiation-associated gene expression networks. Dysregulated SR protein activity and altered splicing patterns are widely implicated in cancer and neurodegenerative disease biology, making SRSF9 a relevant node for mechanistic studies of splicing-driven phenotypes.
SRp30c CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SRSF9 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SRSF9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SRSF9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SRp30c protein expression.
This CRISPR knockout system enables efficient generation of SRSF9-deficient cell models for investigation of SRp30c signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SRSF9 exon(s) critical for SRp30c function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SRSF9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SRp30c CRISPR/Cas9 KO Plasmid (h) and SRp30c CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SRSF9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SRp30c HDR Plasmid (h) and SRp30c HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SRSF9 homology arms to support homology-directed repair at defined SRSF9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.