SRP19 Antibody (G-1): sc-393775
- SRP19 Antibody (G-1) is a mouse monoclonal IgG1 κ SRP19 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 18-49 within an internal region of SRP19 of human origin
- SRP19 Antibody (G-1) is recommended for detection of SRP19 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and porcine
- Anti-SRP19 Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SRP19 Antibody (G-1) for WB applications. These reagents are now offered in bundles with SRP19 Antibody (G-1) (see ordering information below).
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SRP19 Antibody (G-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects SRP19 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRP19 (G-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The signal recognition particle (SRP) is a crucial ribonucleoprotein complex that plays a vital role in targeting nascent proteins to the endoplasmic reticulum (ER) for proper folding and processing. The SRP consists of a unique Alu domain and an S domain, which together contain six proteins, including SRP19, SRP54, SRP68, and SRP72, along with SRP9 and SRP14. SRP19 is essential for SRP complex assembly, as SRP19 binds to free SRP RNA, facilitating the subsequent binding of SRP54, which interacts with both the nascent signal peptide and SRP RNA. This interaction is critical for proper localization of proteins destined for secretion or membrane insertion, ensuring efficient direction to the ER. SRP19, along with SRP68 and SRP72, is localized in the nucleolus and cytoplasm, highlighting the nucleolus as a key site for assembly and interaction of ribonucleoproteins involved in protein synthesis. SRP19′s importance in this process underscores its potential as a target for research into protein targeting mechanisms and related cellular functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SRP19 Antibody (G-1) References:
- Signal recognition particle components in the nucleolus. | Politz, JC., et al. 2000. Proc Natl Acad Sci U S A. 97: 55-60. PMID: 10618370
- The nucleolus and the four ribonucleoproteins of translation. | Pederson, T. and Politz, JC. 2000. J Cell Biol. 148: 1091-5. PMID: 10725320
- Crystal structure of an early protein-RNA assembly complex of the signal recognition particle. | Wild, K., et al. 2001. Science. 294: 598-601. PMID: 11641499
- Isolation and characterization of a cDNA clone encoding the 19 kDa protein of signal recognition particle (SRP): expression and binding to 7SL RNA. | Lingelbach, K., et al. 1988. Nucleic Acids Res. 16: 9431-42. PMID: 2460823
- Subcellular distribution of signal recognition particle and 7SL-RNA determined with polypeptide-specific antibodies and complementary DNA probe. | Walter, P. and Blobel, G. 1983. J Cell Biol. 97: 1693-9. PMID: 6196367
- The uRNA database. | Zwieb, C. 1997. Nucleic Acids Res. 25: 102-3. PMID: 9016512
- Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites. | Gowda, K., et al. 1998. Gene. 207: 197-207. PMID: 9511762
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRP19 Antibody (G-1) | sc-393775 | 200 µg/ml | $322.00 | |||
SRP19 Antibody (G-1): m-IgG Fc BP-HRP Bundle | sc-530575 | 200 µg Ab; 10 µg BP | $361.00 | |||
SRP19 Antibody (G-1): m-IgGκ BP-HRP Bundle | sc-524083 | 200 µg Ab, 40 µg BP | $361.00 | |||
SRP19 Antibody (G-1): m-IgG1 BP-HRP Bundle | sc-544139 | 200 µg Ab; 20 µg BP | $361.00 | |||
SRP19 Antibody (G-1) AC | sc-393775 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SRP19 Antibody (G-1) HRP | sc-393775 HRP | 200 µg/ml | $322.00 | |||
SRP19 Antibody (G-1) FITC | sc-393775 FITC | 200 µg/ml | $336.00 | |||
SRP19 Antibody (G-1) PE | sc-393775 PE | 200 µg/ml | $349.00 | |||
SRP19 Antibody (G-1) Alexa Fluor® 488 | sc-393775 AF488 | 200 µg/ml | $364.00 | |||
SRP19 Antibody (G-1) Alexa Fluor® 546 | sc-393775 AF546 | 200 µg/ml | $364.00 | |||
SRP19 Antibody (G-1) Alexa Fluor® 594 | sc-393775 AF594 | 200 µg/ml | $364.00 | |||
SRP19 Antibody (G-1) Alexa Fluor® 647 | sc-393775 AF647 | 200 µg/ml | $364.00 | |||
SRP19 Antibody (G-1) Alexa Fluor® 680 | sc-393775 AF680 | 200 µg/ml | $364.00 | |||
SRP19 Antibody (G-1) Alexa Fluor® 790 | sc-393775 AF790 | 200 µg/ml | $364.00 | |||
SRP19 (G-1) Neutralizing Peptide | sc-393775 P | 100 µg/0.5 ml | $69.00 |