SRMS Antibody (E-5): sc-376223

SRMS Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects SRMS in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SRMS monoclonal antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SRMS, also known as src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites, is a 488 amino acid nonreceptor tyrosine-protein kinase that plays a crucial role in keratinocyte differentiation and proliferation. SRMS (E-5) antibody recognizes a protein structure that includes one Src homology 3 (SH3) domain, one Src homology 2 (SH2) domain, and one protein kinase domain, which are essential for cellular signaling pathways. The SH3 domain mediates interactions with other proteins, often linking activated growth factors to downstream signaling molecules. This interaction regulates cellular processes, while the SH2 domain binds to tyrosine-phosphorylated proteins, influencing signal transduction. Alterations in the SH3 domain can activate the transforming potential of nonreceptor tyrosine kinases, indicating its role in negative regulation of intrinsic transforming activity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SRMS Antibody (E-5) References:

1. Protein tyrosine kinase 6 negatively regulates growth and promotes enterocyte differentiation in the small intestine.  |  Haegebarth, A., et al. 2006. Mol Cell Biol. 26: 4949-57. PMID: 16782882
2. Substitution mapping in dahl rats identifies two distinct blood pressure quantitative trait loci within 1.12- and 1.25-mb intervals on chromosome 3.  |  Lee, SJ., et al. 2006. Genetics. 174: 2203-13. PMID: 17028336
3. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.  |  Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
4. Nonreceptor tyrosine kinases in prostate cancer.  |  Chang, YM., et al. 2007. Neoplasia. 9: 90-100. PMID: 17357254
5. Signal transduction by receptors with tyrosine kinase activity.  |  Ullrich, A. and Schlessinger, J. 1990. Cell. 61: 203-12. PMID: 2158859
6. Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling.  |  Hunter, T. 1995. Cell. 80: 225-36. PMID: 7834742
7. A novel nonreceptor tyrosine kinase, Srm: cloning and targeted disruption.  |  Kohmura, N., et al. 1994. Mol Cell Biol. 14: 6915-25. PMID: 7935409
8. A role for the epithelial-cell-specific tyrosine kinase Sik during keratinocyte differentiation.  |  Vasioukhin, V. and Tyner, AL. 1997. Proc Natl Acad Sci U S A. 94: 14477-82. PMID: 9405638

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SRMS Antibody (E-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SRMS Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SRMS Antibody (E-5) Specifications

Alternate Product Name: SRMS

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-143

Epitope Target: SRMS

Epitope Species: human

Product Reactivity/Detection: SRMS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SRMS Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SRMS antibody is SRMS Antibody (A-4): sc-374524.