serum reponse factor /SRF Antibody (A-11): sc-25290

SRF Antibody (A-11) is a mouse monoclonal IgM kappa light chain antibody that detects serum response factor (SRF) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRF Antibody (A-11) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SRF (A-11) antibody targets SRF, a transcription factor that binds to the serum response element (SRE) to mediate cellular response to growth stimuli. SRF forms a ternary complex with accessory protein p62TCF at the c-Fos SRE for effective signal transduction, regulating gene expression in response to external signals. SRF also interacts with related Ets domain proteins, including Elk-1 and SRF accessory protein-1 (SAP-1), which share similar DNA binding properties and contribute to ternary complex formation. Post-translational modifications, including phosphorylation at conserved sites within SRF, are critical for functional regulation and activity in various cellular contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

serum reponse factor /SRF Antibody (A-11) References:

1. Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element.  |  Dalton, S. and Treisman, R. 1992. Cell. 68: 597-612. PMID: 1339307
2. Spatial flexibility in ternary complexes between SRF and its accessory proteins.  |  Treisman, R., et al. 1992. EMBO J. 11: 4631-40. PMID: 1425594
3. elk-1 domains responsible for autonomous DNA binding, SRE:SRF interaction and negative regulation of DNA binding.  |  Rao, VN. and Reddy, ES. 1992. Oncogene. 7: 2335-40. PMID: 1437156
4. Human T-cell leukemia virus type 1 Tax enhances serum response factor DNA binding and alters site selection.  |  Winter, HY. and Marriott, SJ. 2007. J Virol. 81: 6089-98. PMID: 17376895
5. Epidermal growth factor and other mitogens induce binding of a protein complex to the c-fos serum response element in human astrocytoma and other cells.  |  Malik, RK., et al. 1991. J Biol Chem. 266: 8576-82. PMID: 1902475
6. The SRE: a growth factor responsive transcriptional regulator.  |  Treisman, R. 1990. Semin Cancer Biol. 1: 47-58. PMID: 2133110
7. The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor.  |  Boxer, LM., et al. 1989. Mol Cell Biol. 9: 515-22. PMID: 2710114
8. Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element.  |  Norman, C., et al. 1988. Cell. 55: 989-1003. PMID: 3203386
9. Functional analysis of a growth factor-responsive transcription factor complex.  |  Hill, CS., et al. 1993. Cell. 73: 395-406. PMID: 8477450

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-serum reponse factor /SRF Antibody (A-11) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-serum reponse factor /SRF Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

serum reponse factor /SRF Antibody (A-11) Specifications

Alternate Product Name: SRF

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 209-508

Epitope Target: SRF

Epitope Species: human

Product Reactivity/Detection: SRF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for serum reponse factor /SRF Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent