SR-β Antibody (D-4): sc-376723
- SR-β Antibody (D-4) is a mouse monoclonal IgG2a κ SR-β antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 239-273 near the C-terminus of SR-β of human origin
- SR-beta Antibody (D-4) is recommended for detection of SR-β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SR-beta Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SR-β Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with SR-β Antibody (D-4) (see ordering information below).
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SR-β Antibody (D-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects SR-beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SR-beta (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SR-beta protein plays a crucial role in protein translocation by targeting nascent polypeptides to the endoplasmic reticulum (ER), which is essential for proper protein folding and maturation. As a component of the signal recognition particle receptor (SRP), which consists of SR-alpha and SR-beta subunits, SR-beta is vital for interaction with ribosomes during cotranslational protein transport. The SRP complex, including GTPases SRP54, SR-alpha, and SR-beta, orchestrates protein delivery to the ER, ensuring correct processing and integration into cellular membranes. Precise regulation of this interaction is critical, as SR-alpha effectively scans membrane-bound ribosomes for signal recognition particle presence, facilitating efficient protein translocation into the ER.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SR-β Antibody (D-4) References:
- The ribosome regulates the GTPase of the beta-subunit of the signal recognition particle receptor. | Bacher, G., et al. 1999. J Cell Biol. 146: 723-30. PMID: 10459008
- Nucleotide-dependent binding of the GTPase domain of the signal recognition particle receptor beta-subunit to the alpha-subunit. | Legate, KR., et al. 2000. J Biol Chem. 275: 27439-46. PMID: 10859309
- The beta-subunit of the protein-conducting channel of the endoplasmic reticulum functions as the guanine nucleotide exchange factor for the beta-subunit of the signal recognition particle receptor. | Helmers, J., et al. 2003. J Biol Chem. 278: 23686-90. PMID: 12750387
- The beta-subunit of the signal recognition particle receptor is a novel GTP-binding protein without intrinsic GTPase activity. | Legate, KR. and Andrews, DW. 2003. J Biol Chem. 278: 27712-20. PMID: 12759365
- An amino-terminal domain containing hydrophobic and hydrophilic sequences binds the signal recognition particle receptor alpha subunit to the beta subunit on the endoplasmic reticulum membrane. | Young, JC., et al. 1995. J Biol Chem. 270: 15650-7. PMID: 7797564
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SR-β Antibody (D-4) | sc-376723 | 200 µg/ml | $316.00 | |||
SR-β Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-523063 | 200 µg Ab, 40 µg BP | $354.00 | |||
SR-β Antibody (D-4): m-IgG2a BP-HRP Bundle | sc-546138 | 200 µg Ab; 10 µg BP | $354.00 | |||
SR-β Antibody (D-4) AC | sc-376723 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SR-β Antibody (D-4) HRP | sc-376723 HRP | 200 µg/ml | $316.00 | |||
SR-β Antibody (D-4) FITC | sc-376723 FITC | 200 µg/ml | $330.00 | |||
SR-β Antibody (D-4) PE | sc-376723 PE | 200 µg/ml | $343.00 | |||
SR-β Antibody (D-4) Alexa Fluor® 488 | sc-376723 AF488 | 200 µg/ml | $357.00 | |||
SR-β Antibody (D-4) Alexa Fluor® 546 | sc-376723 AF546 | 200 µg/ml | $357.00 | |||
SR-β Antibody (D-4) Alexa Fluor® 594 | sc-376723 AF594 | 200 µg/ml | $357.00 | |||
SR-β Antibody (D-4) Alexa Fluor® 647 | sc-376723 AF647 | 200 µg/ml | $357.00 | |||
SR-β Antibody (D-4) Alexa Fluor® 680 | sc-376723 AF680 | 200 µg/ml | $357.00 | |||
SR-β Antibody (D-4) Alexa Fluor® 790 | sc-376723 AF790 | 200 µg/ml | $357.00 | |||
SR-β (D-4) Neutralizing Peptide | sc-376723 P | 100 µg/0.5 ml | $68.00 |