SR-A Antibody (B-9): sc-166139

SR-A (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects SR-A of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-SR-A antibody (B-9) is available as non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. The macrophage class A scavenger receptor (SR-A) mediates the uptake of modified low density lipoprotein (LDL). The gene encoding human SR-A maps to chromosome 8 and gives rise to two alternatively spliced isoforms, type I and II (SR-AI and SR-AII), which were originally cloned from the phorbol ester-treated human monocytic cell line THP-1. Both isoforms contain six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), alpha-helical coiled-coil (IV), collagen-like (V) and a type-specific C-terminal (VI). Domain IV is essential for the trimerization of SR-A, whereas domain V is essential for the wide range of ligand recognition. SR-A is expressed in liver, placenta and brain. Both SR-AI and SR-AII mediate the uptake of LDLs in atherosclerotic lesions. A third isoform, SR-AIII, is unable to uptake LDLs and acts as a dominant negative isoform to possibly protect cells found in advanced atherosclerotic lesions. SR-A plays a role not only in many macrophage-associated pathological processes, including atherosclerosis and Alzheimer′s disease, but also in host defense and as an adhesion molecule.

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SR-A Antibody (B-9) References:

1. Class A scavenger receptor-mediated macrophage adhesion requires coupling of calcium-independent phospholipase A(2) and 12/15-lipoxygenase to Rac and Cdc42 activation.  |  Nikolic, DM., et al. 2007. J Biol Chem. 282: 33405-33411. PMID: 17873277
2. Class A scavenger receptor attenuates myocardial infarction-induced cardiomyocyte necrosis through suppressing M1 macrophage subset polarization.  |  Hu, Y., et al. 2011. Basic Res Cardiol. 106: 1311-28. PMID: 21769674
3. Class A scavenger receptor promotes cerebral ischemic injury by pivoting microglia/macrophage polarization.  |  Xu, Y., et al. 2012. Neuroscience. 218: 35-48. PMID: 22652221
4. Deletion of class A scavenger receptor deteriorates obesity-induced insulin resistance in adipose tissue.  |  Zhu, X., et al. 2014. Diabetes. 63: 562-77. PMID: 24170693
5. Class A scavenger receptor deficiency augments angiotensin II-induced vascular remodeling.  |  Qian, L., et al. 2014. Biochem Pharmacol. 90: 254-64. PMID: 24875449
6. Class A scavenger receptor activation inhibits endoplasmic reticulum stress-induced autophagy in macrophage.  |  Huang, H., et al. 2014. J Biomed Res. 28: 213-21. PMID: 25013404
7. Prostaglandins produced during class A scavenger receptor-mediated macrophage adhesion differentially regulate cytokine production.  |  Nikolic, DM., et al. 2015. J Leukoc Biol. 97: 901-908. PMID: 25717147
8. Cleavage of Type I Collagen by Fibroblast Activation Protein-α Enhances Class A Scavenger Receptor Mediated Macrophage Adhesion.  |  Mazur, A., et al. 2016. PLoS One. 11: e0150287. PMID: 26934296
9. Critical regulation of inflammation via class A scavenger receptor.  |  Xie, L., et al. 2018. Int J Chron Obstruct Pulmon Dis. 13: 1145-1155. PMID: 29695898
10. Exogenous hydrogen sulfide protects fatty liver against ischemia-reperfusion injury by regulating endoplasmic reticulum stress-induced autophagy in macrophage through mediating the class A scavenger receptor pathway in rats.  |  Ruan, Z., et al. 2020. Cell Biol Int. 44: 306-316. PMID: 31483550

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SR-A Antibody (B-9) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-SR-A Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SR-A Antibody (B-9) Specifications

Alternate Product Name: CD204

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 61-250

Epitope Target: SR-A

Epitope Species: human

Product Reactivity/Detection: SR-A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for SR-A Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SR-A antibody is SR-A Antibody (E-5): sc-166184.