SQSTM1/p62 Antibody (D-3): sc-28359

SQSTM1 Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect human SQSTM1 protein, also known as p62, across applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SQSTM1 monoclonal antibody (D-3) targets the amino acid region 151-440 of human SQSTM1 protein, ensuring precise binding and reliable detection. SQSTM1, also called p60, p62, A170, DMRV, OSIL, PDB3, ZIP3, p62B, EBIAP, NADGP, and FTDALS3, plays a critical role in cellular processes such as autophagy, where SQSTM1 acts as a selective autophagy receptor by recognizing and binding to ubiquitinated proteins, facilitating their degradation. Post-translational modifications of SQSTM1, including phosphorylation and ubiquitination, are essential for regulating protein homeostasis and signaling pathways. Phosphorylation of SQSTM1 enhances binding to ubiquitinated targets and autophagic machinery, promoting efficient autophagic flux and preventing protein aggregate accumulation that can lead to cellular dysfunction. Anti-SQSTM1 antibody (D-3) is available in both non-conjugated and various conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing flexibility for diverse experimental setups. SQSTM1 (D-3) antibody is particularly valuable in studying diseases like Paget′s disease of bone, where mutations in the ubiquitin-associated (UBA) domain of SQSTM1 disrupt normal function in aggregate sequestration and cell survival, leading to increased bone resorption and abnormal bone formation. Anti-SQSTM1 antibody (D-3) helps researchers gain deeper insights into molecular mechanisms underlying such conditions, facilitating targeted therapy development and improving understanding of SQSTM1′s role in cellular homeostasis and disease pathology.

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SQSTM1/p62 Antibody (D-3) References:

1. Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease.  |  Hocking, LJ., et al. 2002. Hum Mol Genet. 11: 2735-9. PMID: 12374763
2. Structure of the ubiquitin-associated domain of p62 (SQSTM1) and implications for mutations that cause Paget's disease of bone.  |  Ciani, B., et al. 2003. J Biol Chem. 278: 37409-12. PMID: 12857745
3. Three novel mutations in SQSTM1 identified in familial Paget's disease of bone.  |  Johnson-Pais, TL., et al. 2003. J Bone Miner Res. 18: 1748-53. PMID: 14584883
4. Paget's disease of bone.  |  Rousière, M., et al. 2003. Best Pract Res Clin Rheumatol. 17: 1019-41. PMID: 15123049
5. Identification of SQSTM1 mutations in familial Paget's disease in Australian pedigrees.  |  Good, DA., et al. 2004. Bone. 35: 277-82. PMID: 15207768
6. Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation.  |  Seibenhener, ML., et al. 2004. Mol Cell Biol. 24: 8055-68. PMID: 15340068
7. Etiology of Paget's disease and osteoclast abnormalities.  |  Reddy, SV. 2004. J Cell Biochem. 93: 688-96. PMID: 15389972
8. Structural and functional studies of mutations affecting the UBA domain of SQSTM1 (p62) which cause Paget's disease of bone.  |  Layfield, R., et al. 2004. Biochem Soc Trans. 32: 728-30. PMID: 15493999
9. Ubiquitin-associated domain mutations of SQSTM1 in Paget's disease of bone: evidence for a founder effect in patients of British descent.  |  Lucas, GJ., et al. 2005. J Bone Miner Res. 20: 227-31. PMID: 15647816

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SQSTM1/p62 Antibody (D-3) has 614 citations in a variety of scientific publications.

Primary antibodies like Anti-SQSTM1/p62 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SQSTM1/p62 Antibody (D-3) Specifications

Alternate Product Name: SQSTM1

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 151-440

Epitope Target: SQSTM1

Epitope Species: human

Product Reactivity/Detection: SQSTM1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 614 citations in various scientific publications

Available Conjugates/Forms for SQSTM1/p62 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SQSTM1/p62 antibody is SQSTM1/p62 Antibody (A-6): sc-48402.