SPT5 Antibody (A-3): sc-133097

SPT5 Antibody (A-3) is a mouse monoclonal IgG1 antibody that detects SPT5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPT5, also known as DSIF p160, plays a crucial role in regulating transcriptional elongation, particularly in RNA polymerase II activity. SPT5 is essential for forming the DRB-sensitivity-inducing factor (DSIF) complex when binding to SPT4, which is vital for modulating the transcription process. Notably, SPT5 undergoes significant post-translational modifications, particularly phosphorylation, which affects mobility on SDS-PAGE; SPT5 from mitotic HeLa cells migrates more slowly than from interphase cells due to enhanced phosphorylation. The C-terminal CTR1 domain of SPT5 is specifically targeted for phosphorylation by the positive transcription elongation factor b (P-TEFb), a modification critical for function as a transcriptional elongation regulator. SPT5′s interaction with various transcription factors and RNA polymerase II underscores importance in gene expression regulation, making anti-SPT5 antibody (A-3) an invaluable tool for researchers studying transcriptional mechanisms and cellular processes.

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SPT5 Antibody (A-3) References:

1. A nexus for gene expression-molecular mechanisms of Spt5 and NusG in the three domains of life.  |  Werner, F. 2012. J Mol Biol. 417: 13-27. PMID: 22306403
2. Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.  |  Shetty, A., et al. 2017. Mol Cell. 66: 77-88.e5. PMID: 28366642
3. Targeting Spt5-Pol II by Small-Molecule Inhibitors Uncouples Distinct Activities and Reveals Additional Regulatory Roles.  |  Bahat, A., et al. 2019. Mol Cell. 76: 617-631.e4. PMID: 31564557
4. Spt5-mediated enhancer transcription directly couples enhancer activation with physical promoter interaction.  |  Fitz, J., et al. 2020. Nat Genet. 52: 505-515. PMID: 32251373
5. SPT5 stabilization of promoter-proximal RNA polymerase II.  |  Aoi, Y., et al. 2021. Mol Cell. 81: 4413-4424.e5. PMID: 34480849
6. SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.  |  Hu, S., et al. 2021. Mol Cell. 81: 4425-4439.e6. PMID: 34534457
7. Spt5 histone binding activity preserves chromatin during transcription by RNA polymerase II.  |  Evrin, C., et al. 2022. EMBO J. 41: e109783. PMID: 35102600
8. Spt5 C-terminal repeat domain phosphorylation and length negatively regulate heterochromatin through distinct mechanisms.  |  MacKinnon, S., et al. 2023. PLoS Genet. 19: e1010492. PMID: 37939109
9. Binding of small molecule inhibitors to RNA polymerase-Spt5 complex impacts RNA and DNA stability.  |  Gallardo, A. and Dutagaci, B. 2023. J Comput Aided Mol Des. 38: 1. PMID: 37987925
10. The CDK9-SPT5 Axis in Control of Transcription Elongation by RNAPII.  |  Sun, R. and Fisher, RP. 2024. J Mol Biol. 168746. PMID: 39147127

The preferred SPT5 antibody is SPT5 Antibody (D-3): sc-133217.