SPSY Antibody (A-4): sc-376294
- SPSY Antibody (A-4) is a mouse monoclonal IgG1 κ SPSY antibody provided at 200 µg/ml
- raised against amino acids 202-331 mapping near the C-terminus of SPSY of human origin
- SPSY Antibody (A-4) is recommended for detection of SPSY of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-SPSY Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for SPSY Antibody (A-4) for WB applications. This reagent is now offered in a bundle with SPSY Antibody (A-4) (see ordering information below).
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SEE ALSO...
SPSY Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPSY protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPSY (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPSY protein, also known as spermine synthase, plays a critical role in the biosynthesis of spermine from spermidine, a process vital for cellular growth and differentiation. Spermine, a polyamine found in nearly all living organisms, is essential for maintaining cellular functions and stability. Notably, the regulation of spermine levels is crucial, as a deficiency can lead to increased sensitivity of cells to anti-tumor agents, making spermine synthase a promising target for anti-cancer therapies. Anti-SPSY antibody (A-4) enables researchers to explore the intricate mechanisms of polyamine metabolism and its implications in cancer biology, contributing to the development of novel therapeutic strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPSY Antibody (A-4) References:
- Skin fibroblasts from spermine synthase-deficient hemizygous gyro male (Gy/Y) mice overproduce spermidine and exhibit increased resistance to oxidative stress but decreased resistance to UV irradiation. | Nilsson, J., et al. 2000. Biochem J. 352 Pt 2: 381-7. PMID: 11085931
- Spermine deficiency resulting from targeted disruption of the spermine synthase gene in embryonic stem cells leads to enhanced sensitivity to antiproliferative drugs. | Korhonen, VP., et al. 2001. Mol Pharmacol. 59: 231-8. PMID: 11160858
- Effect of spermine synthase on the sensitivity of cells to anti-tumour agents. | Ikeguchi, Y., et al. 2003. Biochem J. 373: 885-92. PMID: 12737625
- Thirty years of polyamine-related approaches to cancer therapy. Retrospect and prospect. Part 1. Selective enzyme inhibitors. | Seiler, N. 2003. Curr Drug Targets. 4: 537-64. PMID: 14535654
- Spermine is not essential for growth of Saccharomyces cerevisiae: identification of the SPE4 gene (spermine synthase) and characterization of a spe4 deletion mutant. | Hamasaki-Katagiri, N., et al. 1998. Gene. 210: 195-201. PMID: 9573363
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPSY Antibody (A-4) | sc-376294 | 200 µg/ml | $322.00 | |||
SPSY Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-526022 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPSY Antibody (A-4) AC | sc-376294 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SPSY Antibody (A-4) HRP | sc-376294 HRP | 200 µg/ml | $322.00 | |||
SPSY Antibody (A-4) FITC | sc-376294 FITC | 200 µg/ml | $336.00 | |||
SPSY Antibody (A-4) PE | sc-376294 PE | 200 µg/ml | $349.00 | |||
SPSY Antibody (A-4) Alexa Fluor® 488 | sc-376294 AF488 | 200 µg/ml | $364.00 | |||
SPSY Antibody (A-4) Alexa Fluor® 546 | sc-376294 AF546 | 200 µg/ml | $364.00 | |||
SPSY Antibody (A-4) Alexa Fluor® 594 | sc-376294 AF594 | 200 µg/ml | $364.00 | |||
SPSY Antibody (A-4) Alexa Fluor® 647 | sc-376294 AF647 | 200 µg/ml | $364.00 | |||
SPSY Antibody (A-4) Alexa Fluor® 680 | sc-376294 AF680 | 200 µg/ml | $364.00 | |||
SPSY Antibody (A-4) Alexa Fluor® 790 | sc-376294 AF790 | 200 µg/ml | $364.00 |