SPR Antibody (A-11): sc-514777
- SPR Antibody (A-11) is a mouse monoclonal IgG1 κ SPR antibody provided at 200 µg/ml
- raised against amino acids 1-261 representing full length SPR of human origin
- SPR Antibody (A-11) is recommended for detection of SPR of human origin by WB, IP, IF and ELISA
- Anti-SPR Antibody (A-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SPR Antibody (A-11) for WB applications. These reagents are now offered in bundles with SPR Antibody (A-11) (see ordering information below).
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SPR Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPR protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPR Antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPR (sepiapterin reductase) is a homodimeric cytoplasmic protein that plays a crucial role in the biosynthesis of tetrahydrobiopterin (BH4), an essential cofactor for the hydroxylation of aromatic amino acids such as tryptophan, tyrosine, and phenylalanine. This enzymatic activity is vital for the proper synthesis of neurotransmitters, including dopamine, which is critical for maintaining neurological health. Mutations in the gene encoding SPR can lead to sepiapterin reductase deficiency, resulting in a monoamine neurotransmitter deficiency that does not present with hyperphenylalaninemia, and can cause serious conditions such as DOPA-responsive dystonia. Increased expression of SPR mRNA has been observed in the brains of patients with Parkinson′s Disease, indicating a potential involvement of SPR in the pathophysiology of this neurodegenerative disorder. Anti-SPR antibody (A-11) effectively detects and characterizes SPR protein, making this antibody an invaluable tool for research into both normal physiological processes and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPR Antibody (A-11) References:
- Tetrahydrobiopterin deficiencies without hyperphenylalaninemia: diagnosis and genetics of dopa-responsive dystonia and sepiapterin reductase deficiency. | Blau, N., et al. 2001. Mol Genet Metab. 74: 172-85. PMID: 11592814
- Localization of sepiapterin reductase in the human brain. | Ikemoto, K., et al. 2002. Brain Res. 954: 237-46. PMID: 12414107
- Sepiapterin reductase deficiency: clinical presentation and evaluation of long-term therapy. | Echenne, B., et al. 2006. Pediatr Neurol. 35: 308-13. PMID: 17074599
- Dopa-responsive hypersomnia and mixed movement disorder due to sepiapterin reductase deficiency. | Friedman, J., et al. 2006. Neurology. 67: 2032-5. PMID: 17159114
- Sepiapterin reductase expression is increased in Parkinson's disease brain tissue. | Tobin, JE., et al. 2007. Brain Res. 1139: 42-7. PMID: 17270157
- Tetrahydropteridine deficiency impairs mitochondrial function in Dictyostelium discoideum Ax2. | Kim, HL., et al. 2007. FEBS Lett. 581: 5430-4. PMID: 17976377
- A brain-specific decrease of the tyrosine hydroxylase protein in sepiapterin reductase-null mice--as a mouse model for Parkinson's disease. | Takazawa, C., et al. 2008. Biochem Biophys Res Commun. 367: 787-92. PMID: 18201550
- The 1.25 A crystal structure of sepiapterin reductase reveals its binding mode to pterins and brain neurotransmitters. | Auerbach, G., et al. 1997. EMBO J. 16: 7219-30. PMID: 9405351
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPR Antibody (A-11) | sc-514777 | 200 µg/ml | $322.00 | |||
SPR Antibody (A-11): m-IgG Fc BP-HRP Bundle | sc-531220 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPR Antibody (A-11): m-IgGκ BP-HRP Bundle | sc-524843 | 200 µg Ab, 40 µg BP | $361.00 | |||
SPR Antibody (A-11): m-IgG1 BP-HRP Bundle | sc-544440 | 200 µg Ab; 20 µg BP | $361.00 | |||
SPR Antibody (A-11) AC | sc-514777 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SPR Antibody (A-11) HRP | sc-514777 HRP | 200 µg/ml | $322.00 | |||
SPR Antibody (A-11) FITC | sc-514777 FITC | 200 µg/ml | $336.00 | |||
SPR Antibody (A-11) PE | sc-514777 PE | 200 µg/ml | $349.00 | |||
SPR Antibody (A-11) Alexa Fluor® 488 | sc-514777 AF488 | 200 µg/ml | $364.00 | |||
SPR Antibody (A-11) Alexa Fluor® 546 | sc-514777 AF546 | 200 µg/ml | $364.00 | |||
SPR Antibody (A-11) Alexa Fluor® 594 | sc-514777 AF594 | 200 µg/ml | $364.00 | |||
SPR Antibody (A-11) Alexa Fluor® 647 | sc-514777 AF647 | 200 µg/ml | $364.00 | |||
SPR Antibody (A-11) Alexa Fluor® 680 | sc-514777 AF680 | 200 µg/ml | $364.00 | |||
SPR Antibody (A-11) Alexa Fluor® 790 | sc-514777 AF790 | 200 µg/ml | $364.00 |