Spo11 Antibody (C-4): sc-377161
- Spo11 Antibody (C-4) is a mouse monoclonal IgG2b κ Spo11 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 97-396 mapping at the C-terminus of Spo11 of human origin
- Spo11 Antibody (C-4) is recommended for detection of Spo11 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Spo11 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Spo11 Antibody (C-4) for WB applications. This reagent is now offered in a bundle with Spo11 Antibody (C-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Spo11 Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Spo11 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Spo11 antibody (C-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Spo11 protein plays a crucial role in meiotic recombination by generating DNA double-strand breaks, which are essential for proper segregation of chromosomes during meiosis. These breaks predominantly occur in promoter regions where chromatin is more accessible, facilitating necessary interactions for homologous recombination. Expression of Spo11 is primarily localized in testis, aligning with its vital function in initiating meiotic processes. Disruption of Spo11 can lead to significant gonadal abnormalities and infertility due to impaired meiotic progression. Understanding Spo11 function is essential for elucidating mechanisms of genetic diversity and stability, making Spo11 (C-4) monoclonal antibody a valuable tool for researchers studying meiosis and related genetic processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Spo11 Antibody (C-4) References:
- Multiple roles of Spo11 in meiotic chromosome behavior. | Celerin, M., et al. 2000. EMBO J. 19: 2739-50. PMID: 10835371
- [Mechanisms and control of meiotic recombination in the yeast Saccharomyces cerevisiae]. | Mézard, C., et al. 1999. J Soc Biol. 193: 23-7. PMID: 10851552
- Identification and characterization of an SPO11 homolog in the mouse. | Metzler-Guillemain, C. and de Massy, B. 2000. Chromosoma. 109: 133-8. PMID: 10855504
- The mouse Spo11 gene is required for meiotic chromosome synapsis. | Romanienko, PJ. and Camerini-Otero, RD. 2000. Mol Cell. 6: 975-87. PMID: 11106738
- Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11. | Baudat, F., et al. 2000. Mol Cell. 6: 989-98. PMID: 11106739
- The control of Spo11's interaction with meiotic recombination hotspots. | Prieler, S., et al. 2005. Genes Dev. 19: 255-69. PMID: 15655113
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Spo11 Antibody (C-4) | sc-377161 | 200 µg/ml | $322.00 | |||
Spo11 Antibody (C-4): m-IgGκ BP-HRP Bundle | sc-523216 | 200 µg Ab, 40 µg BP | $361.00 | |||
Spo11 Antibody (C-4) AC | sc-377161 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Spo11 Antibody (C-4) HRP | sc-377161 HRP | 200 µg/ml | $322.00 | |||
Spo11 Antibody (C-4) FITC | sc-377161 FITC | 200 µg/ml | $336.00 | |||
Spo11 Antibody (C-4) PE | sc-377161 PE | 200 µg/ml | $349.00 | |||
Spo11 Antibody (C-4) Alexa Fluor® 488 | sc-377161 AF488 | 200 µg/ml | $364.00 | |||
Spo11 Antibody (C-4) Alexa Fluor® 546 | sc-377161 AF546 | 200 µg/ml | $364.00 | |||
Spo11 Antibody (C-4) Alexa Fluor® 594 | sc-377161 AF594 | 200 µg/ml | $364.00 | |||
Spo11 Antibody (C-4) Alexa Fluor® 647 | sc-377161 AF647 | 200 µg/ml | $364.00 | |||
Spo11 Antibody (C-4) Alexa Fluor® 680 | sc-377161 AF680 | 200 µg/ml | $364.00 | |||
Spo11 Antibody (C-4) Alexa Fluor® 790 | sc-377161 AF790 | 200 µg/ml | $364.00 |