SphK2 Antibody (9C5E1): sc-517192
- SphK2 Antibody (9C5E1) is a mouse monoclonal IgG1 κ SphK2 antibody, cited in 1 publications, provided at 100 µg/ml
- raised against a recombinant protein corresponding to amino acids 36-52 of SphK2 of human origin
- recommended for detection of SphK2 of human origin by IF, IHC(P), FCM and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SphK2 Antibody (9C5E1). This work is in progress.
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SphK2 Antibody (9C5E1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the SphK2 protein of human origin by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-SphK2 antibody (9C5E1) is available as a non-conjugated format. Sphingosine kinase 2 (SphK2) plays a crucial role in sphingolipid metabolism, catalyzing sphingosine phosphorylation to produce sphingosine 1-phosphate (S1P), a bioactive lipid integral to various cellular processes. S1P functions as a signaling molecule influencing cell proliferation, survival, and migration, making S1P vital for maintaining cellular homeostasis and responding to external stimuli. SphK2 involvement extends to immune response regulation and potential cancer progression, where altered S1P signaling can contribute to tumor growth and metastasis. SphK2 localizes in the cytoplasm and can translocate to the plasma membrane, significant for signaling pathway function. SphK2 differential expression in various tissues, particularly upregulation in certain cancers, highlights SphK2 potential as a therapeutic target and disease progression biomarker.
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SphK2 Antibody (9C5E1) References:
- Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. | Liu, H., et al. 2000. J Biol Chem. 275: 19513-20. PMID: 10751414
- An oncogenic role of sphingosine kinase. | Xia, P., et al. 2000. Curr Biol. 10: 1527-30. PMID: 11114522
- Sphingosine kinase expression regulates apoptosis and caspase activation in PC12 cells. | Edsall, LC., et al. 2001. J Neurochem. 76: 1573-84. PMID: 11238741
- TNF-alpha-induced sphingosine 1-phosphate inhibits apoptosis through a phosphatidylinositol 3-kinase/Akt pathway in human hepatocytes. | Osawa, Y., et al. 2001. J Immunol. 167: 173-80. PMID: 11418646
- Sphingosine kinase regulates hepatoma cell differentiation: roles of hepatocyte nuclear factor and retinoid receptor. | Osawa, Y., et al. 2001. Biochem Biophys Res Commun. 286: 673-7. PMID: 11520048
- Sphingosine 1-phosphate evokes calcium signals in C2C12 myoblasts via Edg3 and Edg5 receptors. | Meacci, E., et al. 2002. Biochem J. 362: 349-57. PMID: 11853542
- Identification and characterization of RPK118, a novel sphingosine kinase-1-binding protein. | Hayashi, S., et al. 2002. J Biol Chem. 277: 33319-24. PMID: 12077123
- SphK2 over-expression promotes osteosarcoma cell growth. | Xu, D., et al. 2017. Oncotarget. 8: 105525-105535. PMID: 29285269
- New insights into the roles and regulation of SphK2 as a therapeutic target in cancer chemoresistance. | Hasanifard, L., et al. 2019. J Cell Physiol. 234: 8162-8181. PMID: 30456838
- Targeting SphK2 Reverses Acquired Resistance of Regorafenib in Hepatocellular Carcinoma. | Shi, W., et al. 2020. Front Oncol. 10: 694. PMID: 32670862
- Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension. | Ranasinghe, ADCU., et al. 2023. Circ Res. 133: 704-719. PMID: 37698017
- Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes. | Komai, M., et al. 2024. J Lipid Res. 65: 100510. PMID: 38280459
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SphK2 Antibody (9C5E1) | sc-517192 | 100 µg/ml | $322.00 |