SPCA2 Antibody (B-5): sc-398330

SPCA2 (B-5) is a mouse monoclonal IgG1 antibody that detects SPCA2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-SPCA2 antibody (B-5) is available as non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. The family of P-type Ca2+-transport ATPases is made up of three subfamilies: sarco(endo)plasmic-reticulum Ca2+ ATPases (SERCA), plasma-membrane Ca2+ ATPases (PMCA), and secretory-pathway Ca2+ ATPases (SPCA). The SPCA1 protein (encoded for by the ATP2C1 gene) is a Ca2+/Mn2+-transport ATPase. SPCA1 localizes to the Golgi apparatus and, together with SERCA2, maintains the ionic milieu in the Golgi lumen. SPCA2 (encoded by the ATP2C2 gene) also localizes to the Golgi apparatus and has a higher enzymatic turnover rate than SPCA1 while having a high affinity for cytosolic Ca2+. The enzymatic properties of human SPCA2 and the restriction of tissue expression to the gastrointestinal and respiratory tracts, prostate, thyroid, salivary, and mammary glands may define a Ca2+-ATPase pump with a specific physiological role in secretory cells.

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SPCA2 Antibody (B-5) References:

1. A novel isoform of the secretory pathway Ca2+,Mn(2+)-ATPase, hSPCA2, has unusual properties and is expressed in the brain.  |  Xiang, M., et al. 2005. J Biol Chem. 280: 11608-14. PMID: 15677451
2. The secretory pathway Ca2+/Mn2+-ATPase 2 is a Golgi-localized pump with high affinity for Ca2+ ions.  |  Vanoevelen, J., et al. 2005. J Biol Chem. 280: 22800-8. PMID: 15831496
3. Dissection of the functional differences between human secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and 2 isoenzymes by steady-state and transient kinetic analyses.  |  Dode, L., et al. 2006. J Biol Chem. 281: 3182-9. PMID: 16332677

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SPCA2 Antibody (B-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SPCA2 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SPCA2 Antibody (B-5) Specifications

Alternate Product Name: SPCA2

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 925-946

Epitope Target: SPCA2

Epitope Species: human

Product Reactivity/Detection: SPCA2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SPCA2 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred SPCA1/2 antibody is SPCA1/2 Antibody (B-3): sc-377339.